Detection and quantification of dental plaque based on laser-induced autofluorescence intensity ratio values
Detection and quantification of dental plaque based on laser-induced autofluorescence intensity ratio values
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DOI：
10.1117/1.jbo.20.4.048001
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发表时间：
2015-04-01
影响因子：
3.5
通讯作者：
Subhash, Narayanan
中科院分区：
文献类型：
--
作者：
Joseph, Betsy;Prasanth, Chandra Sekhar;Subhash, Narayanan
本研究的目的是评估激光诱导自体荧光（LIAF）光谱检测和定量牙菌斑的适用性。用404 nm半导体激光激发，在300例患者的牙菌斑（0-3级菌斑指数）中原位记录LIAF光谱。根据LIAF光谱数据计算发射峰的荧光强度比，然后绘制其散点图，并计算接收器工作特性下的面积。临床上不可见的1级斑块的LIAF光谱在510 nm处显示出突出的发射峰，在630 nm附近具有卫星峰，而0级斑块在500 nm附近具有单峰。荧光强度比值（F510/F630）随斑块分级的增加而呈下降趋势，不同等级之间的比值差异有统计学意义（p < 0.01）。区分0级和1级斑块的总体灵敏度和特异性均为100%。这项研究的临床意义在于，基于荧光光谱强度比（F510/F630）开发的诊断算法将有助于精确识别微量牙菌斑，而无需公开解决方案，并说服患者需要适当的口腔卫生和家庭护理实践。(C)2015年，美国光电仪器工程师学会（SPIE）
The aim of this study was to evaluate the applicability of laser-induced autofluorescence (LIAF) spectroscopy to detect and quantify dental plaque. LIAF spectra were recorded in situ from dental plaque (0-3 grades of plaque index) in 300 patients with 404 nm diode laser excitation. The fluorescence intensity ratio of the emission peaks was calculated from the LIAF spectral data following which their scatter plots were drawn and the area under the receiver operating characteristics were calculated. The LIAF spectrum of clinically invisible grade-1 plaque showed a prominent emission peak at 510 nm with a satellite peak around 630 nm in contrast to grade 0 that has a single peak around 500 nm. The fluorescence intensity ratio (F510/F630) has a decreasing trend with increase in plaque grade and the ratio values show statistically significant differences (p < 0.01) between different grades. An overall sensitivity and specificity of 100% each was achieved for discrimination between grade-0 and grade-1 plaque. The clinical significance of this study is that the diagnostic algorithm developed based on fluorescence spectral intensity ratio (F510/F630) would be useful to precisely identify minute amounts of plaque without the need for disclosing solutions and to convince patients of the need for proper oral hygiene and homecare practices. (C) 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)