Purification of DNA polymerase II stimulatory factor I, a yeast single-stranded DNA-binding protein.
Purification of DNA polymerase II stimulatory factor I, a yeast single-stranded DNA-binding protein.
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DNA 聚合酶 II 刺激因子 I（一种酵母单链 DNA 结合蛋白）的纯化。
DOI：
10.1073/pnas.87.2.677
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发表时间：
1990
影响因子：
11.1
通讯作者：
Campbell,JL
中科院分区：
文献类型：
--
作者：
Brown,WC;Smiley,JK;Campbell,JL
纯化酵母 DNA 聚合酶 II 时，观察到各种色谱级分含有刺激该聚合酶合成的活性。在本文中，我们报告了其中一种因子——刺激因子 I (SFI) 的纯化和初步表征。 SFI 与 66、37 和 13.5 kDa 三种多肽的明显复合物相关，优先结合单链 DNA，这可能解释了它刺激 DNA 聚合酶 II 的能力。单链 DNA 结合活性与 66 kDa 多肽相关。
Incidental to the purification of yeast DNA polymerase II was the observation that various chromatographic fractions contained activities that stimulated synthesis by this polymerase. In this paper we report the purification and initial characterization of one such factor, stimulatory factor I (SFI). SFI, which is associated with an apparent complex of three polypeptides of 66, 37, and 13.5 kDa, binds preferentially to single-stranded DNA, possibly explaining its ability to stimulate DNA polymerase II. Single-stranded DNA-binding activity is associated with the 66-kDa polypeptide.