Differential Proteomics Analysis of Colonic Tissues in Patients of Slow Transit Constipation.
Differential Proteomics Analysis of Colonic Tissues in Patients of Slow Transit Constipation.
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慢传输性便秘患者结肠组织的差异蛋白质组学分析。
DOI：
10.1155/2016/4814702
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发表时间：
2016
影响因子：
--
通讯作者：
Wu Y
中科院分区：
文献类型：
--
作者：
Wan S;Liu W;Tian C;Ren X;Ding Z;Qian Q;Jiang C;Wu Y
Objective.应用比较蛋白质组学方法，研究STC与正常对照组蛋白质表达的差异。方法.应用双向电泳技术分离5例STC患者和5例正常对照者的标本中的蛋白质。采用计算机辅助图像分析和基质辅助激光解吸电离串联飞行时间质谱（MALDI-TOF-MS）鉴定两组差异表达蛋白。结果在正常对照组的平均凝胶中共鉴定出239个蛋白质点，在STC患者中共鉴定出215个蛋白质点。共匹配197个蛋白点，平均匹配率为82%.有14个蛋白质点与其他蛋白质点表达差异有统计学意义。在这14个蛋白质点中，有12个蛋白质点的表达量显著增加，2个蛋白质点的表达量显著减少。结论STC患者结肠组织蛋白质组学表达与正常对照组差异有统计学意义，可能与STC的发病机制有关。
Objective. To investigate and screen the different expression of proteins in STC and normal group with a comparative proteomic approach. Methods. Two-dimensional electrophoresis was applied to separate the proteins in specimens from both 5 STC patients and 5 normal controls. The proteins with statistically significant differential expression between two groups were identified by computer aided image analysis and matrix assisted laser desorption ionization tandem time of flight mass spectrometry (MALDI-TOF-MS). Results. A total of 239 protein spots were identified in the average gel of the normal control and 215 in patients with STC. A total of 197 protein spots were matched and the mean matching rate was 82%. There were 14 protein spots which were expressed with statistically significant differences from others. Of those 14 protein spots, the expression of 12 spots increased markedly, while that of 2 spots decreased significantly. Conclusion. The proteomics expression in colonic specimens of STC patients is statistically significantly different from that of normal control, which may be associated with the pathogenesis of STC.