Escherichia coli EC93 deploys two plasmid-encoded class I contact-dependent growth inhibition systems for antagonistic bacterial interactions.
Escherichia coli EC93 deploys two plasmid-encoded class I contact-dependent growth inhibition systems for antagonistic bacterial interactions.
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DOI：
10.1099/mgen.0.000534
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发表时间：
2021-03
影响因子：
3.9
通讯作者：
Koskiniemi S
中科院分区：
文献类型：
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作者：
Wäneskog M;Halvorsen T;Filek K;Xu F;Hammarlöf DL;Hayes CS;Braaten BA;Low DA;Poole SJ;Koskiniemi S
接触依赖性生长抑制（CDI）现象和CDI所需的基因（cdiBAI）于2005年从大鼠大肠杆菌分离株（EC 93）中鉴定和分离。虽然cdiBAI EC 93基因座在过去15年中一直是广泛研究的焦点，但对其来源的EC 93分离株知之甚少。在这里，我们对EC 93基因组进行了测序，发现了两个完整的功能性cdiBAI基因座（包括先前鉴定的cdi基因座），它们都携带在一个127 kb的大质粒上。这些cdiBAI系统在实验室培养基中差异表达，使EC 93能够胜过E。缺乏同源cdiI免疫基因的大肠杆菌细胞。两种CDI系统递送不同的效应肽，其各自耗散靶细胞的膜电位，尽管两种毒素显示不同的毒性效力。尽管这些CDI系统的差异表达和毒性效力，都产生了类似的竞争优势，对E。缺乏免疫力的大肠杆菌细胞。这可以通过以下事实来解释：较低表达的cdiBAI系统（cdiBAIEC 93 -2）比高表达的cdiBAIEC 93 -1系统递送更有效的毒素。此外，我们的结果表明，与大多数测序的CDI+细菌分离株不同，E. coliEC 93位于质粒上并在实验室培养基中表达。
The phenomenon of contact-dependent growth inhibition (CDI) and the genes required for CDI (cdiBAI) were identified and isolated in 2005 from an Escherichia coli isolate (EC93) from rats. Although the cdiBAI EC93 locus has been the focus of extensive research during the past 15 years, little is known about the EC93 isolate from which it originates. Here we sequenced the EC93 genome and find two complete and functional cdiBAI loci (including the previously identified cdi locus), both carried on a large 127 kb plasmid. These cdiBAI systems are differentially expressed in laboratory media, enabling EC93 to outcompete E. coli cells lacking cognate cdiI immunity genes. The two CDI systems deliver distinct effector peptides that each dissipate the membrane potential of target cells, although the two toxins display different toxic potencies. Despite the differential expression and toxic potencies of these CDI systems, both yielded similar competitive advantages against E. coli cells lacking immunity. This can be explained by the fact that the less expressed cdiBAI system (cdiBAIEC93-2) delivers a more potent toxin than the highly expressed cdiBAIEC93-1 system. Moreover, our results indicate that unlike most sequenced CDI+ bacterial isolates, the two cdi loci of E. coli EC93 are located on a plasmid and are expressed in laboratory media.
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影响因子：
3.7
作者：
Mercy C;Ize B;Salcedo SP;de Bentzmann S;Bigot S
通讯作者：
Bigot S
影响因子：
4.5
作者：
Ruhe ZC;Nguyen JY;Chen AJ;Leung NY;Hayes CS;Low DA
通讯作者：
Low DA
影响因子：
6.4
作者：
Ruhe ZC;Wallace AB;Low DA;Hayes CS
通讯作者：
Hayes CS
影响因子：
4.5
作者：
Poole SJ;Diner EJ;Aoki SK;Braaten BA;t'Kint de Roodenbeke C;Low DA;Hayes CS
通讯作者：
Hayes CS
影响因子：
64.8
作者：
通讯作者：
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