INVIVO NEUROCHEMICAL EFFECTS OF TAIL PINCH
INVIVO NEUROCHEMICAL EFFECTS OF TAIL PINCH
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DOI：
10.1016/0165-0270(90)90053-i
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发表时间：
1990-09-01
影响因子：
3
通讯作者：
FILLENZ, M
中科院分区：
文献类型：
--
作者：
BOUTELLE, MG;ZETTERSTROM, T;FILLENZ, M
大鼠的夹尾引起了一种特征良好的行为模式，包括啃咬、舔舔和进食。我们已经使用了在体内伏安法和微透析监测神经化学的变化，伴随着行为反应，以5分钟的尾巴捏。夹尾导致细胞外5-羟色胺的增加和海马中5-羟吲哚乙酸的较小和更延迟的增加。在纹状体有一个上升的细胞外多巴胺和抗坏血酸。最近开发的恒电位伏安技术，我们可以连续监测细胞外抗坏血酸的变化。使用这种技术，我们发现了一个非常迅速的上升，抗坏血酸电流在5分钟的尾部捏，电流开始下降，只要夹子被删除。该技术的高时间分辨率也使我们能够记录类似的抗坏血酸的变化，在0.5秒的尾巴捏。
Tail pinch in the rat gives rise to a well characterised pattern of behaviour which includes gnawing, licking and eating. We have used both in vivo voltammetry and microdialysis to monitor neurochemical changes which accompany the behavioural response to a 5-min tail pinch. Tail pinch resulted in a increase of extracellular 5-hydroxytryptamine and a smaller and more delayed increase of 5-hydroxyindole acetic acid in the hippocampus. In the striatum there was a rise of both extracellular dopamine and ascorbate. With a recently developed constant potential voltammetric technique we can continuously monitor changes in extracellular ascorbate. Using this technique we found a very rapid rise in ascorbate current during a 5-min tail pinch; the current began to decline as soon as the clip was removed. The high time resolution of the technique also allowed us to record similar ascorbate changes during a 0.5-s tail pinch.