Genome-wide analysis of heat shock proteins in C4 model, foxtail millet identifies potential candidates for crop improvement under abiotic stress.
Genome-wide analysis of heat shock proteins in C4 model, foxtail millet identifies potential candidates for crop improvement under abiotic stress.
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DOI：
10.1038/srep32641
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发表时间：
2016-09-02
影响因子：
4.6
通讯作者：
Prasad M
中科院分区：
文献类型：
--
作者：
Singh RK;Jaishankar J;Muthamilarasan M;Shweta S;Dangi A;Prasad M
热休克蛋白(HSPs)在提高作物对非生物胁迫的耐受性方面发挥着重要作用。有鉴于此，热休克蛋白及其编码基因在多种植物中都有广泛的特征；然而，了解它们在天然耐逆作物中的结构、组织、进化和表达谱对于确定它们在胁迫响应分子机制中的确切作用是必要的。在此背景下，本研究以C4谷子为模型，分别鉴定了SiHSP100、SiHSP90、SiHSP70、SiHSP60和SisHSP家族的20、9、27、20和37个基因。对这些基因进行了综合分析，并对这些基因在干旱、高温、盐碱和低温胁迫下的表达谱进行了分析，结果表明，在耐逆性不同的谷子品种中，有几个基因显著上调。SisHSP-27在耐热品种中高表达，在酵母系统中的过度表达使其对多种非生物胁迫具有耐受性。对SiHSP基因的甲基化分析表明，在敏感品种中，较高水平的甲基化可能是这些基因在逆境中表达减少的原因。总而言之，这项研究为热休克蛋白在提高压力耐受性中的作用提供了新的线索。
Heat shock proteins (HSPs) perform significant roles in conferring abiotic stress tolerance to crop plants. In view of this, HSPs and their encoding genes were extensively characterized in several plant species; however, understanding their structure, organization, evolution and expression profiling in a naturally stress tolerant crop is necessary to delineate their precise roles in stress-responsive molecular machinery. In this context, the present study has been performed in C4 panicoid model, foxtail millet, which resulted in identification of 20, 9, 27, 20 and 37 genes belonging to SiHSP100, SiHSP90, SiHSP70, SiHSP60 and SisHSP families, respectively. Comprehensive in silico characterization of these genes followed by their expression profiling in response to dehydration, heat, salinity and cold stresses in foxtail millet cultivars contrastingly differing in stress tolerance revealed significant upregulation of several genes in tolerant cultivar. SisHSP-27 showed substantial higher expression in response to heat stress in tolerant cultivar, and its over-expression in yeast system conferred tolerance to several abiotic stresses. Methylation analysis of SiHSP genes suggested that, in susceptible cultivar, higher levels of methylation might be the reason for reduced expression of these genes during stress. Altogether, the study provides novel clues on the role of HSPs in conferring stress tolerance.
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影响因子：
14.9
作者：
Finn RD;Clements J;Arndt W;Miller BL;Wheeler TJ;Schreiber F;Bateman A;Eddy SR
通讯作者：
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影响因子：
5.1
作者：
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作者：
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通讯作者：
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影响因子：
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作者：
HATTON, D;SABLOWSKI, R;BEVAN, M
通讯作者：
BEVAN, M