Spatial distribution of calcite and amorphous calcium carbonate in the cuticle of the terrestrial crustaceans Porcellio scaber and Armadillidium vulgare
Spatial distribution of calcite and amorphous calcium carbonate in the cuticle of the terrestrial crustaceans Porcellio scaber and Armadillidium vulgare
复制标题
DOI：
10.1016/j.jsb.2008.04.010
复制
发表时间：
2008-07-01
影响因子：
3
通讯作者：
Ziegler, Andreas
中科院分区：
文献类型：
--
作者：
Hild, Sabine;Marti, Othmar;Ziegler, Andreas
甲壳动物的角质层是研究矿化生物复合材料性质的一个有趣的模型。角质层由有机基质组成，该有机基质由几丁质-蛋白质纤维和各种数量的结晶和无定形碳酸钙组成。据认为，在等足类动物中，这些矿物质多晶型物的相对量取决于其功能和动物的栖息地。除了成分之外，各种成分的分布也会影响角质层的性质。然而，碳酸钙多晶型物在甲壳动物角质层内的空间分布是未知的。因此，我们分析了矿化铜的陆生等足类Armadillidium vulgare和Porcellio scaber使用扫描电子显微镜，电子探针显微分析和共聚焦μ-拉曼光谱成像。我们第一次表明，矿物相排列在不同的层。方解石仅限于角质层的外层，对应于外角质层。无定形碳酸钙位于位于外角质层下方的内角质层内。在这两个层中，矿物排列成直径约为20 nm的颗粒行。结果表明，功能意义的矿物质分布，符合雅阁和逃避行为的动物。（C）. 2008年爱思唯尔公司All rights reserved.
The crustacean cuticle is an interesting model to study the properties of mineralized bio-composites. The cuticle consists of an organic matrix composed of chitin-protein fibres associated with various amounts of crystalline and amorphous calcium carbonate. It is thought that in isopods the relative amounts of these mineral polymorphs depend on its function and the habitat of the animal. In addition to the composition, the distribution of the various components should affect the properties of the cuticle. However, the spatial distribution of calcium carbonate polymorphs within the crustacean cuticle is unknown. Therefore, we analyzed the mineralized cuticles of the terrestrial isopods Armadillidium vulgare and Porcellio scaber using scanning electron-microscopy, electron probe microanalysis and confocal mu-Raman spectroscopic imaging. We show for the first time that the mineral phases are arranged in distinct layers. Calcite is restricted to the outer layer of the cuticle that corresponds to the exocuticle. Amorphous calcium carbonate is located within the endocuticle that lies below the exocuticle. Within both layers mineral is arranged in rows of granules with diameters of about 20 nm. The results suggest functional implications of mineral distribution that accord to the moulting and escape behaviour of the animals. (C). 2008 Elsevier Inc. All rights reserved.