Molecular analysis of zyg-11, a maternal-effect gene required for early embryogenesis of Caenorhabditis elegans.
Molecular analysis of zyg-11, a maternal-effect gene required for early embryogenesis of Caenorhabditis elegans.
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zyg-11 的分子分析，zyg-11 是秀丽隐杆线虫早期胚胎发生所需的母体效应基因。
DOI：
10.1007/bf00280370
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Kemphues,KJ
中科院分区：
文献类型：
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作者：
Carter,PW;Roos,JM;Kemphues,KJ
在秀丽隐杆线虫的早期胚胎发生过程中，母体作用基因-11的产物是必需的。缺乏功能性zyg-11基因产物的单细胞胚胎表现为减数分裂中期II的停滞，原核形成的延迟，细胞质异常剧烈的运动，多个原核的形成，P颗粒的不正确分离以及第一个卵裂沟的不正确位置。我们分离并测序了一个zyg-11的分子克隆，并证明显微注射克隆的DNA可以挽救ezyg-11的突变。转录分析表明，尽管zyg-11突变具有严格的母系效应表型，但该基因的转录并不局限于雌性种系。
The product of the maternally acting genezyg-11is required for early embryogenesis ofCaenorhabditis elegans. One-cell embryos that lack a functionalzyg-11gene product exhibit an arrest of meiosis at metaphase II, a delay in the formation of pronuclei, unusually vigorous movements of cytoplasm, the formation of multiple pronuclei, incorrect segregation of P granules, and incorrect placement of the first cleavage furrow. We have isolated and sequenced a molecular clone ofzyg-11, and shown that microinjection of the cloned DNA can rescuezyg-11mutations. A transcriptional analysis shows that transcription of the gene is not limited to the female germ-line, despite the strict maternal-effect phenotype ofzyg-11mutations.