Global expression of prophage genes in Escherichia coli O157:H7 strain EDL933 in response to norfloxacin
Global expression of prophage genes in Escherichia coli O157:H7 strain EDL933 in response to norfloxacin
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DOI：
10.1128/aac.49.3.931-944.2005
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发表时间：
2005-03-01
影响因子：
4.9
通讯作者：
Schmidt, H
中科院分区：
文献类型：
--
作者：
Herold, S;Siebert, J;Schmidt, H
我们研究了低浓度的旋转酶抑制剂诺氟沙星对肠出血性大肠杆菌 O157:117 菌株 EDL933 转录组的影响。为此，我们使用了含有大肠杆菌 O157:117 菌株 EDL933 和 RIMD0509952 以及大肠杆菌 K-12 菌株 MG1655 特异性寡核苷酸的商业 DNA 微阵列。在所应用的条件下，可以分析 5,963 个点（占所有点的 94%）。其中，118 个点 (P < 0.05) 表明阵列上存在的大肠杆菌基因转录上调，122 个点 (P < 0.05) 表明转录下调。 85 个上调的 EDL933 基因是噬菌体传播的。其中 52 个可归因于编码志贺毒素的噬菌体（Stx 噬菌体）BP-933W 和 CP-933V；其他 33 个基因属于 EDL933 基因组中的非 Stx 原噬菌体元件。在用诺氟沙星诱导后，在 stxA(2) 的情况下，BP-933W 原噬菌体基因组中存在的基因被最强烈地诱导达 158 倍。另外 22 个上调基因似乎是大肠杆菌 O157:117 菌株 RIMD0509952 特异性噬菌体元件，其余 11 个基因主要与重组和应激功能相关。下调主要针对负责细菌初级代谢的基因，例如能量产生、细胞分裂和氨基酸生物合成。有趣的是，肠上皮细胞消失位点中存在的一些基因似乎被下调。研究结果表明，低浓度的诺氟沙星对大肠杆菌O157:H7的转录组有深远的影响。
We investigated the influence of a low concentration of the gyrase inhibitor norfloxacin on the transcriptome of enterohemorrhagic Escherichia coli O157:117 strain EDL933. For this purpose, we used a commercial DNA microarray containing oligonucleotides specific for E. coli O157:117 strains EDL933 and RIMD0509952 and E. coli K-12 strain MG1655. Under the conditions applied, 5,963 spots (94% of all spots) could be analyzed. Among these, 118 spots (P < 0.05) indicated transcriptional upregulation and 122 spots (P < 0.05) indicated transcriptional downregulation of the E. coli genes present on the array. Eighty-five upregulated EDL933 genes were phage borne. Fifty-two of them could be ascribed to the Shiga toxin-encoding phages (Stx phages) BP-933W and CP-933V; the other 33 genes belonged to non-Stx prophage elements in the EDL933 genome. Genes present in the BP-933W prophage genome were induced most strongly up to 158-fold in the case of stxA(2), upon induction with norfloxacin. Twenty-two additional upregulated genes appeared to be E. coli O157:117 strain RIMD0509952-specific phage elements, and the remaining 11 genes were related mainly to recombination and stress functions. Downregulation was indicated predominantly for genes responsible for bacterial primary metabolism, such as energy production, cell division, and amino acid biosynthesis. Interestingly, some genes present in the locus of enterocyte effacement appeared to be downregulated. The results of the study have shown that a low concentration of norfloxacin has profound effects on the transcriptome of E. coli O157:H7.