METHYLATED BASES IN DNA OF ANIMAL ORIGIN
METHYLATED BASES IN DNA OF ANIMAL ORIGIN
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DOI：
10.1016/0003-9861(70)90328-0
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发表时间：
1970-01-01
影响因子：
3.9
通讯作者：
BROWN, GM
中科院分区：
文献类型：
--
作者：
CULP, LA;DORE, E;BROWN, GM
HeLa细胞、L细胞、人胚肺细胞和鸡胚在甲硫氨酸-甲基-14 C存在下生长。然后分离这些细胞的DNA，并通过测量放射性分析甲基化碱基。在L细胞和鸡胚的DNA中，除了胸腺嘧啶之外，唯一的甲基化碱基是5-甲基胞嘧啶。来自人胚肺细胞的DNA含有5-甲基胞嘧啶和相对少量的3-甲基胞嘧啶。HeLa DNA含有这两个甲基化胞嘧啶加上少量的1-甲基鸟嘌呤、7-甲基鸟嘌呤、N2-甲基鸟嘌呤和N2-二甲基鸟嘌呤。此外，来自HeLa和L细胞的DNA含有未鉴定的甲基化组分，其可以通过链霉蛋白酶处理从DNA中去除。这种未知物质不存在于鸡胚和人类胚胎肺细胞的DNA中。
HeLa cells, L cells, human embryonic lung cells, and chick embryos were allowed to grow in the presence of methionine-methyl-14C. The DNA from these cells was then isolated and analyzed for methylated bases by measurement of radioactivity. The only methylated base, other than thymine, found in DNA from L cells and chick embryos was 5-methylcytosine. DNA from human embryonic lung cells contains 5-methylcytosine and a relatively small amount of 3-methylcytosine. HeLa DNA contains these two methylated cytosines plus small quantities of 1-methylguanine, 7-methylguanine, N2-methylguanine, and N2-dimethylguanine. In addition, DNA from HeLa and L cells contains an unidentified methylated component which can be removed from the DNA by treatment with pronase. This unknown substance is not present in DNA from chick embryos and human embryonic lung cells.