TNF-α induces the transcription factor Egr-1, pro-inflammatory cytokines and cell proliferation in human skin fibroblasts and synovial lining cells
TNF-α induces the transcription factor Egr-1, pro-inflammatory cytokines and cell proliferation in human skin fibroblasts and synovial lining cells
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DOI：
10.1007/s002960050032
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发表时间：
1998-02-01
影响因子：
4
通讯作者：
Eibel, H
中科院分区：
文献类型：
--
作者：
Grimbacher, B;Aicher, WK;Eibel, H
在类风湿关节炎 (RA) 中，滑膜衬里细胞的细胞增殖和金属蛋白酶表达的改变与类风湿关节中 TNF-α 水平的增加有关。我们之前表明 RA 患者的滑膜细胞表达高水平的转录因子 Egr-1。在此，我们报道 TNF-α 能够在人类皮肤成纤维细胞以及 RA 和反应性关节炎患者的滑膜细胞中诱导高 Egr-1 mRNA 水平。此外，我们在体外观察到，当滑膜细胞在 TNF-α 存在下培养时，成纤维细胞增殖显着增加，细胞间接触与血管翳样细胞生长导致的生长抑制消失，以及细胞因子表达模式的改变。
In rheumatoid arthritis (RA) cell proliferation and altered metalloproteinase expression of synovial lining cells are associated with increased levels of TNF-alpha in the rheumatoid joint. We previously showed that synoviocytes of RA patients express high levels of the transcription factor Egr-1. Here we report that TNF-alpha is capable of inducing high Egr-1 mRNA levels in human skin fibroblasts and in synoviocytes from both, RA and reactive arthritis patients. Moreover, we observed in vitro a marked increase in fibroblast proliferation, a loss of growth inhibition by cell-to-cell contact with pannus-like cell growth and an altered cytokine expression pattern when synoviocytes were cultured in presence of TNF-alpha.