Mechanism of commitment to a mating partner in Saccharomyces cerevisiae.
Mechanism of commitment to a mating partner in Saccharomyces cerevisiae.
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DOI：
10.1091/mbc.e22-02-0043
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发表时间：
2022-10-01
影响因子：
3.3
通讯作者：
Lew, Daniel J.
中科院分区：
文献类型：
--
作者：
Jacobs, Katherine C.;Gorman, Olivia;Lew, Daniel J.
许多细胞检测并遵循化学信号的梯度来执行其功能。酵母细胞利用细胞外信息素的梯度来定位交配伴侣，为理解细胞如何解码梯度中的空间信息提供了一个易于处理的模型。为了交配，酵母细胞必须将极性朝向交配伴侣。极性位点是移动的，探索细胞皮层，直到它们到达适当的位置，在那里它们停止移动并“承诺”给伴侣。一个简单的模型来解释承诺假定，高浓度的信息素检测到只有在伴侣细胞的极性网站对齐，并导致极性网站的运动停止。在这里，我们探讨如何酵母细胞回应的合作伙伴，使不同数量的信息素。承诺是令人惊讶的强大的各种信息素水平，排除了简单的模型。我们还测试了适应性途径是否对承诺的鲁棒性负责，但我们的结果表明，缺乏这些途径的细胞仍然能够适应信息素的变化。为了解释这种鲁棒性，我们建议每个交配伴侣的极性位点附近的陡峭信息素梯度将极性位点捕获在适当的位置。
Many cells detect and follow gradients of chemical signals to perform their functions. Yeast cells use gradients of extracellular pheromones to locate mating partners, providing a tractable model for understanding how cells decode the spatial information in gradients. To mate, yeast cells must orient polarity toward the mating partner. Polarity sites are mobile, exploring the cell cortex until they reach the proper position, where they stop moving and “commit” to the partner. A simple model to explain commitment posits that a high concentration of pheromone is detected only upon alignment of partner cells’ polarity sites and causes polarity site movement to stop. Here we explore how yeast cells respond to partners that make different amounts of pheromone. Commitment was surprisingly robust to various pheromone levels, ruling out the simple model. We also tested whether adaptive pathways were responsible for the robustness of commitment, but our results show that cells lacking those pathways were still able to accommodate changes in pheromone. To explain this robustness, we suggest that the steep pheromone gradients near each mating partner’s polarity site trap the polarity site in place.