3D Printed scaffolds with hierarchical biomimetic structure for osteochondral regeneration
3D Printed scaffolds with hierarchical biomimetic structure for osteochondral regeneration
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DOI：
10.1016/j.nano.2019.04.002
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发表时间：
2019-07-01
影响因子：
5.4
通讯作者：
Zhang, Lijie Grace
中科院分区：
文献类型：
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作者：
Zhou, Xuan;Esworthy, Timothy;Zhang, Lijie Grace
由创伤和/或病理性疾病引起的骨软骨缺损是严重的临床问题。由于供体来源不足和植入组织的潜在免疫排斥，目前的方法仍然不能产生令人满意的结果。3D打印技术在制造可定制产品方面表现出了巨大的潜力。仿生组织基质本研究的目的是研究具有仿生双相结构的3D打印支架用于骨软骨再生。为此，纳米羟基磷灰石和转化生长因子β 1纳米颗粒被合成并分别分布到双相支架的下层和上层，该双相支架是使用3D立体光刻打印机制造的。我们的研究结果表明，这种支架设计成功地促进了人骨髓间充质干细胞的成骨和成软骨分化，以及增强了与成骨和成软骨相关的基因表达。这一发现表明，具有仿生双相结构的3D打印骨软骨支架是骨软骨修复和再生的绝佳候选者。(C)2019爱思唯尔公司All rights reserved.
Osteochondral defects resulting from trauma and/or pathologic disorders are critical clinical problems. The current approaches still do not yield satisfactory due to insufficient donor sources and potential immunological rejection of implanted tissues. 3D printing technology has shown great promise for fabricating customizable. biomimetic tissue matrices. The purpose of the present study is to investigate 3D printed scaffolds with biomimetic, biphasic structure for osteochondral regeneration. For this purpose, nano-hydroxyapatite and transforming growth factor beta 1 nanoparticles were synthesized and distributed separately into the lower and upper layers of the biphasic scaffold, which was fabricated using 3D stereolithography printer. Our results showed that this scaffold design successfully promoted osteogenic and chondrogenic differentiation of human bone marrow mesenchymal stem cells, as well as enhanced gene expression associated with both osteogenesis and chondrogenesis alike. The finding demonstrated that 3D printed osteochondral scaffolds with biomimetic, biphasic structure are excellent candidates for osteochondral repair and regeneration. (C) 2019 Elsevier Inc. All rights reserved.