Early genes responsive to abscisic acid during heterophyllous induction in Marsilea quadrifolia
Early genes responsive to abscisic acid during heterophyllous induction in Marsilea quadrifolia
复制标题
DOI：
10.1023/a:1013612331583
复制
发表时间：
2001-12-01
影响因子：
5.1
通讯作者：
Lin, BL
中科院分区：
文献类型：
--
作者：
Hsu, TC;Liu, HC;Lin, BL
水生蕨类植物四叶海芋会因应自然环境和栽培条件的变化而产生不同类型的叶子。当条件有利于产生淹没型叶时，外源施用植物激素脱落酸（阿坝）诱导气生型叶的形成。响应阿坝的组织定位于茎尖分生组织和相关器官原基。从这些组织中，至少鉴定出两层ABA调控的早期基因，包括7个初级基因和17个次级基因。这些基因，指定ABRH为ABA响应的异叶性，表现出不同的表达模式在异叶性诱导过程中。ABRH基因转录水平的变化开始早，在0.5-1.0小时内添加阿坝的培养基。有些变化是短暂的，而另一些则是持久的。ABRH与已知基因具有广泛的序列同源性，包括编码转录因子、蛋白激酶、膜转运蛋白、代谢酶、结构蛋白和叶绿体基因组编码的基因。这些ABRH的鉴定是了解异形调控机制的第一步，结果表明ABA控制的形态发生中涉及新的代谢和调控途径。
The aquatic fern Marsilea quadrifolia produces different types of leaves in response to changes in natural environment and culture conditions. When the conditions are in favor of producing the submerged-type leaves, exogenous application of the plant hormone abscisic acid (ABA) induces the formation of aerial-type leaves. Tissues responsive to ABA were localized to the shoot apical meristem and the associated organ primordia. From these tissues, at least two tiers of ABA-regulated early genes were identified, including seven primary genes and seventeen secondary genes. These genes, designated ABRH for ABA-responsive heterophylly, showed diverse expression patterns during the course of heterophyllous induction. Changes in the transcript level of ABRH genes started early, within 0.5-1.0 h after the addition of ABA to the culture medium. Some changes were transient while the others were persistent. The ABRHs contain extensive sequence homology to known genes, including those encoding transcription factors, protein kinases, membrane transporters, metabolic enzymes, structural proteins and those encoded by the chloroplast genome. Identification of these ABRHs is a first step toward the understanding of the regulation mechanisms of heterophylly, and the results suggest the involvement of novel metabolic and regulatory pathways in ABA-controlled morphogenesis.