Glycophorins A, B, and C: a family of sialoglycoproteins. Isolation and preliminary characterization of trypsin derived peptides.
Glycophorins A, B, and C: a family of sialoglycoproteins. Isolation and preliminary characterization of trypsin derived peptides.
复制标题
血型糖蛋白 A、B 和 C：唾液酸糖蛋白家族。
DOI：
--
复制
发表时间：
1978
期刊：
影响因子：
--
通讯作者：
H. Furthmayr
中科院分区：
文献类型：
--
作者：
H. Furthmayr
采用二碘水杨酸锂萃取、苯酚水溶液分割、洗涤剂凝胶过滤、十二烷基硫酸钠制备聚丙烯酰胺凝胶电泳等方法，从红细胞膜中分离到两个新的唾液糖蛋白：糖蛋白B和糖蛋白C。通过氨基酸和碳水化合物分析、胰蛋白酶肽分离、各多肽链氨基末端糖肽的分离纯化，对这两种蛋白进行了表征。糖蛋白B在正常红细胞膜的电泳图中有两种形式，分别为单体和二聚体，糖蛋白a也有类似的描述。方法是使用糖蛋白a羧基末端决定因素的抗体，并用抗体和葡萄球菌的125i蛋白a直接染色凝胶。金黄色葡萄球菌，以及通过二维免疫电泳，只有两种已知形式的糖蛋白A可以检测到。这些数据证实并扩展了人类红细胞中的唾液糖蛋白是二聚体分子的概念，这些二聚体分子要么是在膜中预先形成的，要么是在体外容易产生的。只有糖蛋白A和糖蛋白C在完整红细胞中对胰蛋白酶敏感。
Two new sialoglycoproteins, glycophorin B and glycophorin C, were isolated from erythrocyte membranes by extraction with lithium diiodosalicylate, partition in aqueous phenol, gel filtration in detergent, and preparative polyacrylamide gel electrophoresis in sodium dodecyl sulfate. The two proteins were characterized by amino acid and carbohydrate analysis, separation of tryptic peptides, and isolation and purification of the amino terminal glycopeptide from each polypeptide chain. Glycophorin B is found in two forms in electrophoretograms of normal erythrocyte membranes, corresponding to monomer and dimer, as has been similarly described for glycophorin A. By using antibodies to a carboxy terminal determinant of glycophorin A, and direct staining of gels with antibodies and 125I-protein A from Staph. aureus, as well as by two-dimensional immunoelectrophoreis, only the two known forms of glycophorin A are detectable. The data confirm and extend the notion that the sialoglycoproteins in human red cells are dimeric molecules which are either preformed in the membrane or which can readily be generated in vitro. Only glycophorin A and glycophorin C are sensitive to trypsin while in situ in the intact red blood cells.