Diacyllipid micelle-based nanocarrier for magnetically guided delivery of drugs in photodynamic therapy
Diacyllipid micelle-based nanocarrier for magnetically guided delivery of drugs in photodynamic therapy
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DOI：
10.1021/mp060015p
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发表时间：
2006-07-01
影响因子：
4.9
通讯作者：
Prasad, Paras N.
中科院分区：
文献类型：
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作者：
Cinteza, Ludmila O.;Ohulchanskyy, Tymish Y.;Prasad, Paras N.
我们报告了一种新型多功能聚合物胶束纳米载体系统的设计、合成和表征，该系统展示了磁导向药物递送与光激活光动力疗法的组合功能。具体而言，纳米载体由二酰基磷脂-聚乙二醇（PE-PEG）与光敏剂药物2-[1-己氧基乙基]-2-脱乙烯基焦脱镁叶绿酸-a（HPPH）共聚合的聚合物胶束和磁性Fe 3 O 4纳米颗粒组成。纳米载体在几周内显示出优异的稳定性和活性。通过透射电子显微镜和光学光谱进行了物理化学表征。一个有效的细胞摄取已证实与共聚焦激光扫描显微镜。HPPH的负载效率实际上不受磁性纳米颗粒的影响，并且保留了其光毒性。纳米载体的磁响应通过其在体外磁性定向递送至肿瘤细胞来证明。对细胞摄取的磁泳控制提供增强的成像和光毒性。这些多功能纳米载体展示了纳米化学为靶向光动力治疗提供的令人兴奋的前景。
We report the design, synthesis using nanochemistry, and characterization of a novel multifunctional polymeric micelle-based nanocarrier system, which demonstrates combined function of magnetophoretically guided drug delivery together with light-activated photodynamic therapy. Specifically, the nanocarrier consists of polymeric micelles of diacylphospholipid-poly-(ethylene glycol) (PE-PEG) coloaded with the photosensitizer drug 2-[1-hexyloxyethyl]-2-devinyl pyropheophorbide-a (HPPH), and magnetic Fe3O4 nanoparticles. The nanocarrier shows excellent stability and activity over several weeks. The physicochemical characterizations have been carried out by transmission electron micrography and optical spectroscopy. An efficient cellular uptake has been confirmed with confocal laser scanning microscopy. The loading efficiency of HPPH is practically unaffected upon coloading with the magnetic nanoparticles, and its phototoxicity is retained. The magnetic response of the nanocarriers; was demonstrated by their magnetically directed delivery to tumor cells in vitro. The magnetophoretic control on the cellular uptake provides enhanced imaging and phototoxicity. These multifunctional nanocarriers demonstrate the exciting prospect offered by nanochemistry for targeting photodynamic therapy.