Simultaneous detection of eight food allergens using optical thin-film biosensor chips.
Simultaneous detection of eight food allergens using optical thin-film biosensor chips.
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DOI：
10.1021/jf200933b
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发表时间：
2011-06
影响因子：
6.1
通讯作者：
Wei Wang;Jianxun Han;Yajun Wu;Fei Yuan;Ying Chen;Yi-qiang Ge
中科院分区：
文献类型：
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作者：
Wei Wang;Jianxun Han;Yajun Wu;Fei Yuan;Ying Chen;Yi-qiang Ge
食物过敏是当今重要的食品安全问题。为了维护过敏反应患者的安全，许多国家都要求贴上标签，并需要有效可靠的检测方法。本文报道了一种新的方法，通过使用硅基光学薄膜生物传感器芯片，颜色变化的结果可以被肉眼感知，无需任何额外的设备，快速识别食物过敏原。整个系统可同时检测大豆、小麦、花生、腰果、虾、鱼、牛肉、鸡肉等8种食物过敏原。该方法灵敏度高，特异性强，绝对检测限为0.5pg腰果DNA，实际检测限为0.001%。生物传感器芯片检测时间为PCR扩增后约30 min。该检测方法是一种灵敏、特异、高通量、即用型的分析工具，可用于检测或确认8种食物过敏原的存在。
Food allergies are important food safety issues nowadays. To maintain the safety of people who experience allergic reactions, labeling is required in many countries and efficient and reliable detection methods are necessary. This paper reports a novel method for the rapid identification of food allergens through the use of a silicon-based optical thin-film biosensor chip with which color change results can be perceived by the naked eye without any extra equipment. The whole system can detect eight food allergens including soybean, wheat, peanut, cashew, shrimp, fish, beef, and chicken simultaneously. Sensitive and specific detection of the absolute detection limit of this method was 0.5 pg of cashew DNA, and the practical detection limit of 0.001%. The biosensor chip detection time was about 30 min after PCR amplification. The assay is proposed as a sensitive, specific, high-throughput, and ready-to-use analytical tool to detect the presence or confirm the absence of eight food allergens.