Localization of epidermal-type fatty acid binding protein in macrophages in advanced atretic follicles of adult mice
Localization of epidermal-type fatty acid binding protein in macrophages in advanced atretic follicles of adult mice
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DOI：
10.1007/s10735-005-9005-6
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发表时间：
2005-09-01
影响因子：
3.2
通讯作者：
Kondo, Hisatake
中科院分区：
文献类型：
--
作者：
Nourani, Mohammad Reza;Owada, Yuji;Kondo, Hisatake
用免疫光镜和电镜技术研究了表皮型脂肪酸结合蛋白（E-FABP）在小鼠成熟卵巢中的定位。大量巨噬细胞免疫阳性的抗E-FABP和F4/80抗体，连同免疫阴性细胞，发现在先进的闭锁卵泡偏心腔畸形的卵子。虽然一些E-FABP免疫阳性巨噬细胞呈蜘蛛状，并出现单一的，其他人，特别是接近管腔，是圆形和体积大，往往是聚集的。巨大的巨噬细胞含有各种大小的吞噬体，它们被认为是积极参与吞噬凋亡颗粒细胞的巨噬细胞。E-FABP免疫阳性的巨噬细胞及其突起常与邻近的免疫阴性细胞并列，其中一些排列在含有畸形卵的管腔内。另一方面，闭锁卵泡中的E-FABP免疫阴性细胞分为两类：一种是少数，其特征在于含有非管状嵴的小线粒体，推测代表残留的颗粒细胞，而另一种优势类型的特征在于含有管状嵴的大线粒体，推测代表最初围绕闭锁卵泡的卵泡膜细胞。E-FABP-免疫阳性选择性地出现在闭锁卵泡的巨噬细胞中，提示E-FABP和/或其配体脂肪酸可能参与了卵泡闭锁的过程，并且它使得对具有腔室闭塞的晚期闭锁卵泡的鉴定更加可靠，这可以提供比以前更详细的卵泡闭锁的组织学特征。
The localization of epidermal-type fatty acid binding protein (E-FABP) in the mature mouse ovary was examined by immuno-light and electron microscopy. Numerous macrophages immunopositive for both anti-E-FABP and F4/80 antibodies, together with immunonegative cells, were found in advanced atretic follicles that had eccentric lumens containing deformed ova. While some E-FABP-immunopositive macrophages were spider in shape and appeared singly, others, especially close to the lumen, were round and voluminous and tended to be aggregated. The voluminous macrophages contained phagosomes of various sizes and they were regarded as those actively involved in the phagocytosis of apoptotic granulosa cells. E-FABP-immunopositive macrophages and their processes were often apposed to adjacent immunonegative cells, and some of them lined the lumen containing deformed ova. On the other hand, E-FABP-immunonegative cells in the atretic follicles were classified into two types: the one, a minority, was characterized by small mitochondria containing non-tubular cristae and presumably represented residual granulosa cells, while the other dominant type was characterized by large mitochondria containing tubular cristae and presumably represented theca cells originally surrounding the follicles to be atretic. The present detection of E-FABP-immunopositivity selectively in macrophages of the atretic follicles suggests possible involvement of E-FABP and/or its ligand fatty acids in the process of follicular atresia, and it makes more reliable the identification of the advanced atretic follicles with the antral spaces obliterated, which could provide further details on the histology of the follicular atresia than before.