Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry
Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry
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DOI：
10.1039/b910053a
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发表时间：
2009-01-01
期刊：
影响因子：
6.1
通讯作者：
Morgan, Hywel
中科院分区：
文献类型：
--
作者：
Holmes, David;Pettigrew, David;Morgan, Hywel
微型高速无标记细胞分析系统尚未开发，但有潜力提供快速，廉价和简单的全血细胞分析系统，可在临床实践中常规使用。我们展示了一个微流控单细胞阻抗细胞仪，执行白色血细胞分类计数。该装置由一个微流控芯片和微电极组成，可以测量两个频率下单细胞的阻抗。用皂苷/甲酸处理以溶解红细胞的人血流过该装置，并在几分钟内进行全血细胞计数。通过同时测量来自CD抗体缀合细胞的荧光来进行细胞介电参数的验证。这使得来自单个细胞的阻抗信号与表型直接相关。对患者样本的检测显示，与商业（光学/Coulter）血液分析设备的相关性为95%，证明了阻抗微量血细胞计数器用于即时血液分析系统的潜在临床实用性。
Miniature high speed label-free cell analysis systems have yet to be developed, but have the potential to deliver fast, inexpensive and simple full blood cell analysis systems that could be used routinely in clinical practice. We demonstrate a microfluidic single cell impedance cytometer that performs a white blood cell differential count. The device consists of a microfluidic chip with micro-electrodes that measure the impedance of single cells at two frequencies. Human blood, treated with saponin/formic acid to lyse erythrocytes, flows through the device and a complete blood count is performed in a few minutes. Verification of cell dielectric parameters was performed by simultaneously measuring fluorescence from CD antibody-conjugated cells. This enabled direct correlation of impedance signals from individual cells with phenotype. Tests with patient samples showed 95% correlation against commercial (optical/Coulter) blood analysis equipment, demonstrating the potential clinical utility of the impedance microcytometer for a point-of-care blood analysis system.