The regulatory role of Hyper-IL-6 in the differentiation of myeloid and erythroid progenitors derived from human cord blood
The regulatory role of Hyper-IL-6 in the differentiation of myeloid and erythroid progenitors derived from human cord blood
复制标题
Hyper-IL-6 在人脐带血骨髓和红系祖细胞分化中的调节作用
DOI：
10.1016/j.cellimm.2006.07.008
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发表时间：
2006-05-01
影响因子：
4.3
通讯作者：
Fan, Li
中科院分区：
文献类型：
--
作者：
Yu, Zhaocai;Liu, Wenchao;Fan, Li
本研究以人脐血CD 34（+）CD 38（-）细胞为靶细胞，采用无血清液体悬浮培养系统，探讨可溶性白细胞介素6受体（sIL-6 R）与白细胞介素6（IL-6）融合蛋白（Hyper-IL-6）对人髓系和红系祖细胞分化的调控作用。我们发现Hyper-IL-6促进CD 36（-）CD 15（-）CD 14（-）CD 1a（-）IL-6 R（+）髓系祖细胞向CD 15（+）粒细胞和CD 14（+）单核细胞分化，抑制CD 14（-）CD 1a（+）树突状细胞的分化。相反，CD 34（+）CD 38（-）细胞衍生的早期红系祖细胞IL-6 R表达阴性。高IL-6增强了IL-6 R（-）早期红系祖细胞产生CD 36（+）血型糖蛋白A（高）成熟红系细胞。我们的结果表明，Hyper-IL-6促进了CD 15（+）粒细胞、CD 14（+）单核细胞和CD 36（+）血型糖蛋白A（高）细胞的生成，并抑制了CD 14（-）CD 1a（+）树突状细胞的生成。(c)2006年爱思唯尔公司所有权利均受尊重。
This study was designed to investigate the regulatory role of soluble interleukin-6 receptor (sIL-6R) and interleukin-6 (IL-6) fusion protein (Hyper-IL-6) in the differentiation of human myeloid and erythroid progenitors by a serum-free liquid suspension culture system, using the human cord blood-derived CD34(+)CD38(-) cells as a target. We found that Hyper-IL-6 promoted the generation of CD15(+) granulocytic and CD14(+) monocytic cells and suppressed that of CD14(-)CD1a(+) dendritic cells from CD36(-)CD15(-)CD14(-)CD1a(-)IL-6R(+) myeloid progenitors. Conversely, CD34(+)CD38(-) cell-derived early erythroid progenitors were negative for IL-6R expression. Hyper-IL-6 potentiated the generation of CD36(+)glycophorinA(high) mature erythroid cells from the IL-6R(-) early erythroid progenitors. Our results indicate that Hyper-IL-6 augments the generation of CD15(+) granulocytic, CD14(+) monocytic and CD36(+)glycophorinA(high) cell and suppresses that of CD14(-)CD1a(+) dendritic cells. (c) 2006 Elsevier Inc. All rights reseved.