XENOGENEIC MONOCLONAL ANTIBODIES TO MOUSE LYMPHOID DIFFERENTIATION ANTIGENS
XENOGENEIC MONOCLONAL ANTIBODIES TO MOUSE LYMPHOID DIFFERENTIATION ANTIGENS
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DOI：
10.1111/j.1600-065x.1979.tb00289.x
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发表时间：
1979-01-01
影响因子：
8.7
通讯作者：
HERZENBERG, LA
中科院分区：
文献类型：
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作者：
LEDBETTER, JA;HERZENBERG, LA
异种免疫具有检测广泛的抗原决定簇的优势，因为许多常见的蛋白质在进化过程中发生了显著的分化，因此在其他物种中是抗原性的。然而，异种反应的广泛性意味着它们产生的抗血清通常是复杂的，需要广泛的吸收才能使它们对单一抗原具有特异性。这个问题现在已经通过产生产生单抗的杂交瘤来克服(Kohler&Milstein，1975)。这使得可以解剖异种反应，从而可以获得大量的个体抗体，每个抗体只识别广泛反应的常规抗血清识别的一个决定因素。Williams等人(1977)用大鼠细胞免疫小鼠后获得的杂交瘤单抗研究了大鼠淋巴细胞亚群上存在的大鼠细胞表面抗原，即分化抗原。Springer等人(1978a)和Stern等人(1978)使用类似的方法研究小鼠淋巴细胞抗原。他们通过用小鼠淋巴细胞免疫大鼠来制备单抗，并表明这些单抗识别以前未检测到的小鼠细胞表面决定因素，包括似乎是巨噬细胞特异性的糖蛋白抗原(Springer等人。1978b)。Trowbridge(1978)还利用大鼠抗小鼠免疫产生了针对非多形性淋巴细胞表面抗原T200的单抗。
Xenogeneic immunizations have the advantage of detecting a wide range of antigenic determinants because many commonly occurring proteins have diverged significantly during the course of evolution and are thus antigenic in other species. The broadness of xenogeneic responses, however, means that the antisera they produce are usually complex and require extensive absorptions to make them specific for a single antigen. This problem has now been overcome by generating hybridomas producing monoclonal antibodies (Kohler & Milstein 1975). These permit dissection ofthe xenogeneic response so that large amounts of individual antibodies can be obtained, each of which recognizes only one of the determinants recognized by a broadly reactive conventional antiserum. Williams et al.(1977) used hybridoma monoclonal antibodies obtained after immunizations of mice with rat cells to study rat cell-surface antigens present on subpopulations of rat lymphocytes, ie, differentiation antigens. Springer et al.(1978a) and Stern et al.(1978) used a similar approach to study mouse lymphocyte antigens. They prepared monoclonal antibodies by immunizing rats with mouse lymphocytes and showed that these monoclonals recognized previously undetected mouse cell surface determinants including a glycoprotein antigen that appears to be specific for macrophages (Springer et al. 1978b). Trowbridge (1978) also used rat anti-mouse immunizations to generate a monoclonal antibody against the non-polymorphic lymphocyte surface antigen T200.