Chromaffin Progenitor Cells from the Adrenal Medulla
Chromaffin Progenitor Cells from the Adrenal Medulla
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DOI：
10.1007/s10571-010-9571-3
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发表时间：
2010-11-01
影响因子：
4
通讯作者：
Bornstein, Stefan R.
中科院分区：
文献类型：
--
作者：
Ehrhart-Bornstein, Monika;Vukicevic, Vladimir;Bornstein, Stefan R.
肾上腺髓质的嗜铬细胞是交感神经肾上腺谱系的神经嵴细胞。不同的证据表明，即使在成年状态下，也存在增殖能力强的祖细胞亚群。在肾上腺中发现交感肾上腺祖细胞将大大提高对肾上腺生理学及其在肾上腺发病中的潜在作用的认识。在培养中分离和分化这些祖细胞将为了解它们在体外的发育提供工具。此外，由于与交感神经元的密切关系，这些细胞可能为神经退行性疾病的细胞治疗提供一个可扩展的细胞来源。因此，我们的目标是建立有效的分离、富集和在培养中向多巴能神经元分化的染色质祖细胞的方案。
Chromaffin cells of the adrenal medulla are neural crest-derived cells of the sympathoadrenal lineage. Different lines of evidence suggest the existence of a subpopulation of proliferation-competent progenitor cells even in the adult state. The identification of sympathoadrenal progenitors in the adrenal would greatly enhance the understanding of adrenal physiology and their potential role in adrenal pathogenesis. Isolation and differentiation of these progenitor cells in culture would provide a tool to understand their development in vitro. Furthermore, due to the close relation to sympathetic neurons, these cells might provide an expandable source of cells for cell therapy in the treatment of neurodegenerative diseases. We therefore aim to establish protocols for the efficient isolation, enrichment and differentiation of chromaffin progenitor cells to dopa-minergic neurons in culture.