Increased grain dormancy in white-grained wheat by introgression of preharvest sprouting tolerance QTLs
Increased grain dormancy in white-grained wheat by introgression of preharvest sprouting tolerance QTLs
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DOI：
10.1007/s10681-006-9231-3
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发表时间：
2006-12-01
期刊：
影响因子：
1.9
通讯作者：
Miura, H.
中科院分区：
文献类型：
--
作者：
Kottearachchi, N. S.;Uchino, N.;Miura, H.
白粒小麦品种对穗发芽的抗性一直被认为不如红粒小麦品种。之前在高度休眠的日本红小麦Zenkoujikomugi（Zen）中鉴定了两个控制籽粒休眠的QTL QPhs.ocs-3A.1（QPhs-3AS）和QPhs.ocs-4A.1（QPhs-4AL）。以提高白粒小麦的抗小穗病性为目的，利用Zen与白粒小麦Spica杂交构建的40个重组自交系（RILs）构建白粒小麦群体，检测了这2个QTL的渐渗效应。从同一杂交组合中随机培育出20个红粒重组自交系作为对照。在田间和温室中种植RILs，通过发芽试验来评估籽粒休眠。利用与QPhs-3AS和QPhs-4AL紧密连锁的SSR标记对QTL位点进行检测。RIL的休眠变化与籽粒颜色和QPhs-3AS等位基因的差异显著相关。虽然在与QPhs-4AL紧密连锁的SSR标记中检测到等位变异，但Zen-allele和Spica-allele组之间的发芽数据没有差异。正如预期的那样，在QPhs-3AS处具有Zen等位基因的红色颗粒RILs是最休眠的。一些白粒RIL与Zen等位基因在QPhs-3AS表现出更高的休眠相比，红粒RIL与替代等位基因。这些结果表明，QPhs-3AS基因的渐渗可能有助于提高白粒小麦的籽粒休眠性。
White-grained wheat cultivars have long been recognized to be less resistant to preharvest sprouting (PHS) than the red-grained ones. Previously two QTLs for grain dormancy, QPhs.ocs-3A.1 (QPhs-3AS) and QPhs.ocs-4A.1 (QPhs-4AL) were identified in a highly dormant Japanese red wheat, Zenkoujikomugi (Zen). Aiming at improvement of PHS tolerance in white-grained wheat, the introgression effect of these two QTLs in a white-grained population consisting of 40 recombinant inbred lines (RILs) developed from a cross between Zen and white-grained Spica was examined here. Random 20 RILs with red grains were also developed from the same cross and used as a control population. The RILs were grown in the field and in the glasshouse to evaluate the grain dormancy by germination test. Several SSR markers closely linked to the QPhs-3AS and QPhs-4AL were used to estimate the alleles at the QTLs. Dormancy variation in the RILs was significantly associated with the differences for grain color and the alleles at QPhs-3AS over several years. Although allelic variation was detected in a SSR marker closely linked to QPhs-4AL there was no difference in germination data between the Zen-allele and the Spica-allele groups. As expected, the red-grained RILs with the Zen allele at QPhs-3AS were the most dormant. Some white-grained RILs with the Zen allele at QPhs-3AS showed higher dormancy compared to the red-grained RILs with the alternative allele. These results demonstrated that introgression of the QPhs-3AS gene could contribute to the increased grain dormancy in white-grained wheat.