Simultaneous injection of bone marrow cells and stromal cells into bone marrow accelerates hematopoiesis in vivo
Simultaneous injection of bone marrow cells and stromal cells into bone marrow accelerates hematopoiesis in vivo
复制标题
DOI：
10.1634/stemcells.2004-0173
复制
发表时间：
2004-01-01
期刊：
影响因子：
5.2
通讯作者：
Ikehara, S
中科院分区：
文献类型：
--
作者：
Zhang, YM;Adachi, Y;Ikehara, S
我们以前已经证明，基质细胞可以支持造血细胞在体外和体内的增殖和分化，并且造血干细胞和基质细胞之间存在主要组织相容性复合物限制。我们还发现，骨髓内（IBM）注射同种异体骨髓细胞（BMC）比静脉注射更快地重建造血细胞。我们研究了同时注射基质细胞和BMCs到同一骨髓中对供体造血细胞恢复的影响，并证明同时注射BMCs和基质细胞在与静脉内注射BMCs+基质细胞或单独IBM注射BMCs相比，用供体造血细胞重建受体。
We have previously demonstrated that stromal cells can support the proliferation and differentiation of hematopoietic cells in vitro and in vivo and that a major histocompatibility complex restriction exists between hematopoietic stem cells and stromal cells. We have also found that intra-bone marrow (IBM) injection of allogeneic bone marrow cells (BMCs) leads to more rapid reconstitution of hematopoietic cells than intravenous injection.In the present study, we examine the effect of simultaneous injection of stromal cells and BMCs into the same bone marrow on the recovery of donor hematopoietic cells and demonstrate that simultaneous IBM injection of BMCs plus stromal cells is more effective in reconstituting recipients with donor hematopoietic cells than intravenous injection of BMCs plus stromal cells or IBM injection of BMCs alone.