PDMS-Zwitterionic Hybrid for Facile, Antifouling Microfluidic Device Fabrication
PDMS-Zwitterionic Hybrid for Facile, Antifouling Microfluidic Device Fabrication
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PDMS-两性离子杂化物用于简便、防污微流体装置的制造
DOI：
10.1021/acs.langmuir.1c03375
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发表时间：
2022
期刊：
影响因子：
3.9
通讯作者：
Wagner, William R.
中科院分区：
文献类型：
--
作者：
Mercader, Anthony;Ye, Sang-Ho;Kim, Seungil;Orizondo, Ryan A.;Cho, Sung Kwon;Wagner, William R.
聚二甲基硅氧烷（PDMS）由于其生物稳定性、细胞相容性、透气性和光学特性，已被广泛用于生物医学设备和医学研究。然而，PDMS的一些性质产生了严重的限制，特别是通过蛋白质和细胞粘附的污染。在这项研究中，二烯丙基封端的磺基甜菜碱（SB-二烯丙基）分子的合成，然后直接与商业PDMS基地（Sylgard 184）和固化剂混合，以产生两性离子基团轴承PDMS（PDMS-SB）的混合物，不需要复杂的或额外的表面改性过程所需的最终产品。在暴露于新鲜绵羊血后的体外血小板行为检查显示，与PDMS对照相比，PDMS-SB混合表面的血小板沉积显著减少（p<0.05，n = 5）。使用合成的聚合物通过软光刻的可制造性被认为是可比较的未改性的PDMS。确认通过O2等离子体处理的结合，并测量强度，再次发现与对照相当。PDMS-SB微流体装置被成功地制造，并且显示出改善的血液相容性，相对于PDMS对照装置，这可以减少由于凝块形成而导致的通道阻塞。此外，还用概念验证微通道装置测试了通过PDMS-SB杂化膜的气体（CO2）转移，并显示出与通过PDMS对照的气体（CO2）转移相当。
Poly(dimethylsiloxane) (PDMS) has been used in a wide range of biomedical devices and medical research due to its biostability, cytocompatibility, gas permeability, and optical properties. Yet, some properties of PDMS create critical limitations, particularly fouling through protein and cell adhesion. In this study, a diallyl-terminated sulfobetaine (SB-diallyl) molecule was synthesized and then directly mixed with a commercial PDMS base (Sylgard 184) and curing agent to produce a zwitterionic group-bearing PDMS (PDMS–SB) hybrid that does not require a complex or an additional surface modification process for the desired end product. In vitro examination of antifouling behavior following exposure to fresh ovine blood showed a significant reduction in platelet deposition for the PDMS–SB hybrid surface compared to that of a PDMS control (p< 0.05,n= 5). The manufacturability via soft lithography using the synthesized polymers was found to be comparable to that for unmodified PDMS. Bonding via O2plasma treatment was confirmed, and the strength was measured and again found to be comparable to the control. PDMS–SB microfluidic devices were successfully fabricated and showed improved blood compatibility that could reduce channel occlusion due to clot formation relative to PDMS control devices. Further, gas (CO2) transfer through a PDMS–SB hybrid membrane was also tested with a proof-of-concept microchannel device and shown to be comparable to that through the PDMS control.