Wnt signals mediate a fate decision between otic placode and epidermis
Wnt signals mediate a fate decision between otic placode and epidermis
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DOI：
10.1242/dev.02271
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发表时间：
2006-03-01
期刊：
影响因子：
4.6
通讯作者：
Groves, AK
中科院分区：
文献类型：
--
作者：
Ohyama, T;Mohamed, OA;Groves, AK
耳基板，内耳的原基，从以转录因子Pax 2的表达为特征的外胚层区域发育而来。以前的命运定位研究表明，这些Pax 2（+）细胞将产生耳基板组织和表皮，但将Pax 2（+）场分为基板和表皮区域的信号是未知的。我们报道了Wnt信号在Pax 2（+）细胞的一个亚群中被正常激活，并且这些细胞中β-连环蛋白的条件性失活导致以耳基板为代价的表皮标志物的扩增。相反，Pax 2（+）细胞中β-连环蛋白的条件性激活导致耳基板以表皮为代价的扩张，并且所得耳组织仅表达背侧耳囊标记物。总之，这些结果表明，Wnt信号传导的作用是引导Pax 2（+）细胞的耳基板，而不是表皮，命运和促进耳囊背细胞的身份。
The otic placode, the anlagen of the inner ear, develops from an ectodermal field characterized by expression of the transcription factor Pax2. Previous fate mapping studies suggest that these Pax2(+)cells will give rise to both otic placode tissue and epidermis, but the signals that divide the Pax2(+) field into placodal and epidermal territories are unknown. We report that Wnt signaling is normally activated in a subset of Pax2(+) cells, and that conditional inactivation of p-catenin in these cells causes an expansion of epidermal markers at the expense of the otic placode. Conversely, conditional activation of P-catenin in Pax2(+) cells causes an expansion of the otic placode at the expense of epidermis, and the resulting otic tissue expresses exclusively dorsal otocyst markers. Together, these results suggest that Wnt signaling acts instructively to direct Pax2(+) cells to an otic placodal, rather than an epidermal, fate and promotes dorsal cell identities in the otocyst.