Sleep, brain energy levels, and food intake: Relationship between hypothalamic ATP concentrations, food intake, and body weight during sleep-wake and sleep deprivation in rats.
Sleep, brain energy levels, and food intake: Relationship between hypothalamic ATP concentrations, food intake, and body weight during sleep-wake and sleep deprivation in rats.
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DOI：
10.1007/s11818-011-0524-y
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发表时间：
2011-06
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影响因子：
--
通讯作者：
Basheer R
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文献类型：
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作者：
Dworak M;Kim T;McCarley RW;Basheer R
饥饿和进食的感觉是一种依赖于清醒状态的行为，由下丘脑内的特定中心调节。虽然室旁核（PVN），弓状核（ARC），背侧和腹内侧下丘脑（DMH/VMH）调节进食，外侧下丘脑（LH）与进食和觉醒/REM睡眠调节。为了研究睡眠和觉醒对食物摄入和体重的影响，我们还测量了下丘脑ATP浓度，这是已知的进食行为和睡眠-觉醒调节。在大鼠中，在24小时光暗循环期间和通过温和处理进行的6小时睡眠剥夺（SD）期间测量食物摄入量和体重。从PVN，ARC/DMH/VMH，LH的组织样品收集后6小时的SD和时间匹配的昼夜控制。ATP的测定采用荧光素酶生物发光法。在整个24小时光暗期间，大鼠消耗约28.13±4.48 g食物，增加5.22±1.65 g，食物摄入量与体重呈正相关。在SD期间，与未受干扰的对照组相比，虽然食物摄入量显着增加+147.31 ± 6.13%，但体重显着减轻（-93.29 ± 13.64%）。SD组仅LH区ATP含量显著降低（-44.60 ~21.13%），PVN、ARC/DMH/VMH区无明显变化。结果表明，在LH和个人的食物摄入量的ATP浓度之间有很强的整体相关性，并建议睡眠-觉醒依赖的神经元控制的食物摄入量和体重。
The feeling of hunger and feeding, a wake–state-dependent behavior, is regulated by specific centers within the hypothalamus. While paraventricular nucleus (PVN), arcuate nucleus (ARC), and dorso- and ventromedial hypothalamus (DMH/VMH) regulate feeding, the lateral hypothalamus (LH) is associated both with feeding and wake/REM sleep regulation. In order to examine the effects of sleep and wakefulness on food intake and body weight, we also measured hypothalamic ATP concentrations, which are known to be involved in feeding behavior and sleep–wake regulation. In rats, food intake and body weight was measured during a 24-h light–dark cycle and during 6 h of sleep deprivation (SD) performed by gentle handling. Tissue samples from the PVN, ARC/DMH/VMH, and LH were collected after 6 h of SD and from time-matched diurnal controls. ATP was measured by luciferin-luciferase bioluminescence assay. Across the 24-h light–dark period, rats consumed approximately 28.13±4.48 g of food and gained 5.22±1.65 g with a positive correlation between food intake and body weight. During SD, while food intake increased significantly +147.31±6.13%, they lost weight significantly (–93.29±13.64%) when compared to undisturbed controls. SD resulted in a significant decrease in ATP levels only in LH (–44.60±21.13%) with no change in PVN, ARC/DMH/VMH region when compared with undisturbed controls. The results indicate a strong overall correlation between ATP concentrations in the LH and individual food intake and suggest a sleep–wake dependent neuronal control of food intake and body weight.