In vitro effects of metal ions on cellular metabolism and the correlation between these effects and the uptake of the ions.
In vitro effects of metal ions on cellular metabolism and the correlation between these effects and the uptake of the ions.
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DOI：
10.1002/jbm.820280404
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发表时间：
1994-04
期刊：
影响因子：
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通讯作者：
J. Wataha;C. T. Hanks;R. G. Craig
中科院分区：
文献类型：
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作者：
J. Wataha;C. T. Hanks;R. G. Craig
研究了Ag+1、Au+3、Cd+2、Cu+2、Ga+3、In+3、Ni+2、Pd+2和Zn+2对哺乳动物成纤维细胞DNA合成、蛋白质合成、琥珀酸脱氢酶活性和细胞总蛋白的影响。这些细胞功能对金属离子暴露的反应速率进行了比较，并与离子进入细胞的吸收速率相关。这些反应率有显着差异：DNA合成下降最快，其次是蛋白质合成，琥珀酸脱氢酶活性，和总蛋白。对于大多数金属离子，这种响应顺序是相似的。在4小时，金属离子的摄取率与琥珀酸脱氢酶活性的抑制最密切相关，而在8小时，摄取与蛋白质合成的抑制最密切相关。细胞对所有金属离子的类似反应可能意味着这些离子通过类似的机制作用于细胞。银+1，铜+2，和锌+2的摄取率足够快，在体内暴露于这些金属的组织的时间少于12小时将能够破坏细胞代谢。
The effects of Ag+1, Au+3, Cd+2, Cu+2, Ga+3, In+3, Ni+2, Pd+2, and Zn+2 on DNA synthesis, protein synthesis, succinic dehydrogenase activity, and total cellular protein of mammalian fibroblasts were measured for exposures less than 12 h. The rates at which these cellular functions responded to metal ion exposure were compared and related to the uptake rate of the ions into the cells. These rates of response were significantly different: DNA synthesis decreased the fastest, followed by protein synthesis, succinic dehydrogenase activity, and total protein. This order of response was similar for most metal ions. At 4 h, the rate of uptake of the metal ions correlated most closely with depression of succinic dehydrogenase activity, whereas at 8 h, the uptake correlated most closely with depression of protein synthesis. The similar response of cells to all metal ions may imply that these ions act on cells by similar mechanisms. The rates of uptake of Ag+1, Cu+2, and Zn+2 were sufficiently fast that in vivo exposures of tissues to these metals for periods less than 12 h would be capable of disrupting cellular metabolism.