Examination of trophic discrimination factors and lipid corrections in muscle, ovary and mucus tissues of ayu (<i>Plecoglossus altivelis altivelis</i>) using carbon and nitrogen stable isotope analyses
Examination of trophic discrimination factors and lipid corrections in muscle, ovary and mucus tissues of ayu (<i>Plecoglossus altivelis altivelis</i>) using carbon and nitrogen stable isotope analyses
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使用碳和氮稳定同位素分析检查香鱼（<i>Plecoglossus altivelis altivelis</i>）肌肉、卵巢和粘液组织的营养辨别因子和脂质校正
DOI：
10.11369/jji.17-017
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
丸山 敦
中科院分区：
文献类型：
--
作者：
沢田 隼;米倉 竜次;丸山 敦
碳和氮稳定同位素比率(分别为δ13C和δ15N)已被广泛用于评估动物的食物习性，其基础是动物与其食物之间的同位素比率的恒定差异，即营养(饮食-组织)区分因子。然而，最近的研究发现，后者在不同组织或物种之间的差异比以前认识到的更大。在水族箱实验中，用同位素相同的食物(混合良好的商品鱼食)养殖日本典型的淡水渔业资源--阿鱼(Plecoglossus Altivelis Altivelis)，从而获得肌肉组织、卵巢组织和表皮粘液特有的TDF值，从而能够估计脂肪含量对δ13C值的影响。在肌肉组织中，去脂样品比未处理样品高0.8‰-5.6‰，并依赖于整体C：N比，而卵巢组织中的脂肪含量及其影响是恒定的。根据已报道的模型，通过对体积C/N比的回归，得到了AYU肌肉组织特有的脂肪校正方程。肌肉组织(2.4‰)、卵巢组织(1.9‰)和表皮粘液(1.0‰)的TDF值不同。肌肉组织(2.5‰)、卵巢组织(1.6‰)和表皮粘液(1.5‰)的TDF值也不同。本研究中所建立的AYU的TDF和脂质校正方程能够准确地估计该物种的食物习性和迁徙生态。
Carbon and nitrogen stable isotope ratios (δ 13 C and δ 15 N, respectively) have been widely used to estimate food habits of animals, based on constant differences in isotope ratios between such animals and their diets, namely, trophic (diet–tissue) discrimination factors (TDF). However, recent studies have found the latter to vary more between different tissues or species than recognized previously. An aquarium experiment, in which ayu (Plecoglossus altivelis altivelis), a representative Japanese freshwater fishery resource, were cultivated with isotopically identical food (well-mixed commercial fish food), thereby obtaining TDF specific to muscle tissue, ovarian tissue and epidermal mucus, enabled estimation of the effect of lipid content on δ 13 C values. That in muscle tissue was 0.8‰–5.6‰ higher in lipid-eliminated samples than in untreated ones and was dependent on bulk C: N ratios, whereas the lipid content and its effect were constant in ovarian tissue. Lipid correction equations specific to muscle tissue of ayu were obtained by regressions on bulk C: N ratio, according to previously reported models. Lipid-free TDF in δ 13 C values differed between muscle tissue (2.4‰), ovarian tissue (1.9‰) and epidermal mucus (1.0‰). TDF in δ 15 N values also differed between muscle tissue (2.5‰), ovarian tissue (1.6‰) and epidermal mucus (1.5‰). The TDF and lipid correction equations for ayu demonstrated in this study enable accurate estimation of food habits and migration ecology of the species.