Analysis of 16S-23S rRNA internal transcribed spacer regions in Pasteurellaceae isolated from laboratory rodents
Analysis of 16S-23S rRNA internal transcribed spacer regions in Pasteurellaceae isolated from laboratory rodents
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DOI：
10.1016/j.mimet.2012.06.013
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发表时间：
2012-09-01
影响因子：
2.2
通讯作者：
Sager, Martin
中科院分区：
文献类型：
--
作者：
Benga, Laurentiu;Benten, W. Peter M.;Sager, Martin
对从啮齿动物中分离到的嗜肺巴氏杆菌Jawetz和Heyl、[放线杆菌]Muris、流感嗜血杆菌和Bisgaard分类群17个成员的内转录间隔区(ITS)进行了研究，并分析了它们用于区分这些物种的可行性。所分析的所有物种的参考菌株都显示出独特的物种特异性ITS图谱，这在49株[P.]临床分离株中进一步存在。嗜气单胞菌生物型Jawetz和Heyl和[A.]Muris允许通过与参考菌株模式比较来鉴定它们。对扩增片段的序列分析表明，除“流感嗜血杆菌”外，较大的ITSile+Ala含有tRNA(Ile(GAU))和tRNA(Ala(UGC))基因，较小的ITSglu含有tRNA(Glu(Uuc))基因。“流感嗜血杆菌”发现了两个分别较大和较小的ITS片段。每种ITS类型的长度和序列在[P.]嗜气单胞菌生物型Jawetz和Heyl和[A.]检测了穆里斯菌株。相反地。ITS序列在种间差异显著，ITSile+Ala的同源性为61.2%~89.5%，ITSglu的同源性为56.5%~86.8%。确定了种间差异显著但种内高度保守的序列区域，可用于设计探针用于种水平上的啮齿动物巴氏杆菌科的鉴定。(C)2012爱思唯尔B.V.保留所有权利。
The internal transcribed spacer (ITS) regions of members of Pasteurellaceae isolated from rodents, including the [Pasteurella] pneumotropica biotypes Jawetz and Heyl, [Actinobacillus] muris, "Hemophilus influenzaemurium" and Bisgaard taxon 17 were studied and their feasibility to discriminate these species was analyzed. The reference strains of all species analyzed showed unique species-specific ITS patterns which were further present in 49 clinical isolates of [P.] pneumotropica biotypes Jawetz and Heyl and [A.] muris allowing their identification by comparison to the reference strains pattern. Sequence analysis of the amplified fragments revealed in all species, with exception of "H. influenzaemurium", a larger ITSile+ala which contained the genes for tRNA(Ile(GAU)) and tRNA(Ala( UGC)) and a smaller ITSglu with the tRNA(Glu(UUC)) gene. "H. influenzaemurium" revealed two each of the larger and respectively the smaller ITS fragments. Both the length and the sequence of each ITS type were highly conserved within the [P.] pneumotropica biotypes Jawetz and Heyl and [A.] muris strains tested. On the contrary. ITS sequences revealed significant interspecies variations with identity levels ranging from 61.2 to 89.5% for ITSile+ala and 56.5 to 86.8% for ITSglu. Sequences regions with significant interspecies variation but highly conserved within the species were identified and might be used to design probes for the identification of rodent Pasteurellaceae to the species level. (C) 2012 Elsevier B.V. All rights reserved.