Isolation and Identification of Antiarthritic Constituents from Glycine tabacina and Network Pharmacology-Based Prediction of Their Protective Mechanisms against Rheumatoid Arthritis
Isolation and Identification of Antiarthritic Constituents from Glycine tabacina and Network Pharmacology-Based Prediction of Their Protective Mechanisms against Rheumatoid Arthritis
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DOI：
10.1021/acs.jafc.0c00878
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发表时间：
2020-09-30
影响因子：
6.1
通讯作者：
He, Chengwei
中科院分区：
文献类型：
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作者：
Tu, Yanbei;Wang, Kai;He, Chengwei
甘氨酸烟叶(Labill.)是民间治疗类风湿性关节炎(RA)的食用中草药。目前对这种干燥草本植物的植物化学研究导致分离出8个新的Coumestans，命名为Glytabastan A-H(1-8)，以及23个已知化合物9-31。用波谱方法对它们的结构进行了鉴定。结果表明，在体外，Coumestans 1-6和8-10具有抑制关节炎症的作用，而Coumestans 1、2、9和10显著抑制核因子受体激活剂kappa B配体(RANKL)诱导的破骨细胞生成。此外，网络药理学分析表明，烟粉抗RA作用涉及多靶点、多途径，如PI3K/Akt和MAPK信号通路，以及炎症反应和细胞因子介导的信号通路等多种生物学过程。这些结果表明，该物种及其新的古美斯坦类化合物可以作为潜在的功能食品或医药用途的抗关节炎药物。
Glycine tabacina (Labill.) Benth is an edible medicinal herb for rheumatoid arthritis (RA) treatment in folk medicine. Current phytochemical research on this dried herb led to the isolation of eight new coumestans, named glytabastan A-H (1-8), and twenty-three known compounds 9-31. Their structures were elucidated using spectroscopic methods. The antiarthritic activities of all isolates were evaluated, and the results showed that coumestans 1-6 and 8-10 could inhibit arthritic inflammation in vitro, while coumestans 1, 2, 9, and 10 significantly blocked the osteoclastogenesis induced by receptor activator of nuclear factor (NF) kappa B ligand (RANKL). Moreover, network pharmacological analysis revealed that the anti-RA effect of G. tabacina involved multitargets, multipathways such as PI3K/Akt and MAPK signaling pathways, and various biological processes such as inflammatory response and cytokine-mediated signaling pathways. These results suggested that this species and its novel coumestans could serve as potential antiarthritic agents for functional food or medicinal use.