Glutamate clearance mechanisms in resected cortical dysplasia
Glutamate clearance mechanisms in resected cortical dysplasia
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DOI：
10.3171/2010.10.jns10715
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发表时间：
2011-04-01
影响因子：
4.1
通讯作者：
Najm, Imad
中科院分区：
文献类型：
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作者：
Gonzalez-Martinez, Jorge A.;Ying, Zhong;Najm, Imad
Object.谷氨酸转运体（GLT）表达的变化可能在致痫性的表达中起作用。先前的研究表明，在人类发育不良的神经元中，神经元GLT的数量增加。尚未研究球囊细胞（BC）和含BC的皮质发育不良中神经胶质和神经元GLT和谷氨酰胺合成酶（GS）的表达。作者分析了5例皮质发育不良诱导的药物难治性局灶性癫痫患者切除的新皮质样本，这些患者接受了术中长时间皮质电图（ECoG）记录。免疫组化法检测5例脑组织中胶质细胞（GLT 1/EAAT 2）和神经细胞（EAAT 3、EAAC 1）GLT和GS蛋白的表达。作者还评估了原位共定位的GLT和GS与神经元和神经胶质标记。在长时间的术中ECoG记录中，含有皮质发育不良病变的球囊细胞未表现出发作模式。在BCs和发育不良的神经元中，胶质细胞和神经元GLT的表达存在差异：大多数BCs高表达胶质细胞GLT，但不表达神经元GLT。发育不良的神经元表现出增加的免疫组化染色与神经元EAAT 3，但不与EAAT 2/GLT 1。此外，胶质细胞酸性蛋白阳性的BCs也表达GS。GLT在异型增生和球囊细胞中的表达存在差异。球囊细胞皮质发育不良中存在胶质GLT和GS提示BCs可能具有抗癫痫作用，这与GLT 1缺陷动物中报告的致癫痫性增加一致。(DOI：10.3171/2010.10.JNS10715）
Object. Changes in the expression of glutamate transporters (GLTs) may play a role in the expression of epileptogenicity. Previous studies have shown an increased number of neuronal GLTs in human dysplastie neurons. The expression of glial and neuronal GLTs and glutamine synthetase (GS) in balloon cells (BCs) and BC-containing cortical dysplasia has not been studied.Methods. The authors analyzed neocortical samples that were resected in 5 patients who had cortical dysplasia induced medically intractable focal epilepsy and who underwent extraoperative prolonged electrocorticographic (ECoG) recordings. The expressions of glial (GLT1/EAAT2) and neuronal (EAAT3, EAAC1) GLTs and GS proteins were immunohistochemically studied in all 5 resected samples. The authors also assessed in situ colocalization of GLTs and GS with neuronal and glial markers.Results. Balloon cell containing cortical dysplasia lesions did not exhibit ictal patterns on prolonged extraoperative ECoG recordings. There was a differential expression of glial and neuronal GLTs in BCs and dysplastic neurons: the majority of BCs highly expressed glial but not neuronal GLTs. Dysplastic neurons showed increased immunohistochemical staining with neuronal EAAT3 but not with EAAT2/GLT1. Moreover, only glial fibrillary acidic protein positive BCs also expressed GS.Conclusions. There is a differential GLT expression in dysplastic and balloon cells. The presence of glial GLTs and GS in balloon cell cortical dysplasia suggests a possible antiepileptie role for BCs and is consistent with the reported increased epileptogenicity in GLT1-deficient animals. (DOI: 10.3171/2010.10.JNS10715)