DIAGNOSTIC VALUE OF EXOSOMAL MIRNAS FOR COLORECTAL CANCER
DIAGNOSTIC VALUE OF EXOSOMAL MIRNAS FOR COLORECTAL CANCER
复制标题
DOI：
10.33878/2073-7556-2018-0-2-25-31
复制
发表时间：
2018-06
期刊：
影响因子：
--
通讯作者：
R. Samsonov;M. Tarasov;V. Burdakov;T. Shtam;A. Guljaev;O. Tkachenko;E. Rybakov;M. Filatov;A. Aigner;A. Malek
中科院分区：
文献类型：
--
作者：
R. Samsonov;M. Tarasov;V. Burdakov;T. Shtam;A. Guljaev;O. Tkachenko;E. Rybakov;M. Filatov;A. Aigner;A. Malek
**目的**：评估外泌体微小核糖核酸（microRNA）分析在结直肠癌（CRC）中的诊断意义。
**材料与方法**：采集100例结肠肿瘤患者和20名健康供体的血浆样本。通过差速超速离心法分离外泌体，并采用动态光散射（DLS）、冷冻电子显微镜（cryo - EM）和流式细胞术进行分析。通过逆转录聚合酶链反应（RT - PCR）对外泌体微小核糖核酸进行定量分析。运用克氏 - 瓦氏（Kraskel - Wallis）统计检验和受试者工作特征曲线（ROC）分析来评估所得结果。
**结果**：研究表明，结直肠癌会导致多种外泌体微小核糖核酸浓度发生特征性变化。提出将“相互作用微小核糖核酸对”分析作为结直肠癌个性化诊断的算法。获得了微小核糖核酸对“miR - 223 / miR - 181a”的最佳诊断参数值（敏感度 = 0.93，特异度 = 0.88）。
**结论**：外泌体微小核糖核酸分析为结直肠癌的早期诊断/筛查提供了一种有前景的方法。
OBJECTIVE. Assessment of diagnostic significance of exosomal microRNAs analysis in colorectal cancer (CRC). MATERIALS AND METHODS. Plasma samples from 100 patients with colon tumors and 20 healthy donors. Exosomes were isolated by differential ultracentrif ugation the analysis was performed by dynamic light scattering (DLS), cryo-EM, flow cytometry. Quantitative analysis of exosomal microRNAs was performed by RT-PCR. To evaluate the results obtained, the Kraskel-Wallis statistical test and ROC analysis were used. RESULTS. It is shown that CRC causes characteristic changes in the concentration of a number of exosomal microRNAs. Analysis of «reciprocal miRNAs pairs» was proposed as algorithmfor personalized diagnostic of CRC. The optimal parameters of diagnostic values were obtained for miRNA pair«miR-223 / miR-181a» (sensitivity =0.93, specificity =0.88). CONCLUSION. The analysis of exosomal microRNAs presents a promising method for early diagnostics / screening of colorectal cancer.