Somatic mutation in a cultured mouse myeloma cell affects antigen binding.
Somatic mutation in a cultured mouse myeloma cell affects antigen binding.
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培养的小鼠骨髓瘤细胞中的体细胞突变会影响抗原结合。
DOI：
10.1073/pnas.79.4.1240
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发表时间：
1982
影响因子：
11.1
通讯作者：
Scharff,MD
中科院分区：
文献类型：
--
作者：
Cook,WD;Rudikoff,S;Giusti,AM;Scharff,MD
S107 小鼠骨髓瘤细胞系合成结合半抗原磷酸胆碱的 IgA 抗体，其重链和轻链可变区序列与 BALB/c 小鼠响应磷酸胆碱免疫产生的主要抗体相似（即使不相同）。该细胞系经常自发地产生体细胞变体，产生结合抗原的能力降低的免疫球蛋白。这里描述了一种这样的变体，S 107.U1。当该变体连接到载体上时，其结合磷酸胆碱的能力降低，尽管其对游离半抗原的亲和力与亲本相同。抗原结合的这种减少与 JH 片段中第五个残基处的单个氨基酸取代有关。
The S107 mouse myeloma cell line synthesizes an IgA antibody that binds the hapten phosphocholine and is similar if not identical in its heavy and light chain variable region sequence to the predominant antibody produced by BALB/c mice in response to immunization with phosphocholine. This cell line frequently and spontaneously generates somatic variants producing immunoglobulins with decreased ability to bind antigen. One such variant, S 107.U1, is described here. This variant has a decreased ability to bind phosphocholine when it is attached to a carrier, although its affinity for free hapten is the same as that of the parent. This decrease in antigen binding is associated with a single amino acid substitution at the fifth residue in the JH segment.