Production of functional sperm by subcutaneous auto-grafting of immature testes in rainbow trout
Production of functional sperm by subcutaneous auto-grafting of immature testes in rainbow trout
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DOI：
10.1002/mrd.22949
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发表时间：
2018-02-01
影响因子：
2.5
通讯作者：
Yoshizaki, Goro
中科院分区：
文献类型：
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作者：
Hayashi, Makoto;Sakuma, Daika;Yoshizaki, Goro
性成熟的个体对于育种计划是不可或缺的。鲑鱼需要很长一段时间才能达到性成熟，因此我们的目标是通过将未成熟的睾丸碎片移植到成熟的受体中来缩短获得功能性精子所需的时间，我们预测这将使移植的睾丸碎片跳过漫长的青春期前时期。首先，我们证明了成功的皮下自体移植睾丸碎片在虹鳟鱼。采用单侧睾丸切除术，将游离的未成熟睾丸碎片沿受体鱼背部沿着移植。移植的睾丸碎片与留在体腔内的受体睾丸同步发育，最终都产生了功能性精子。接下来，将未成熟的睾丸碎片自体移植到性成熟雄性的皮下空间中。我们实现了这一点，没有免疫排斥反应，通过分离和冷冻保存睾丸从未成熟的鱼，饲养这些单侧睾丸切除的鱼，直到性成熟。然后将冷冻保存的睾丸自体移植到原来的，现在正在授精的鱼中。移植后5个月内，未成熟睾丸碎片分化并产生功能性精子。通过将这种移植方法与避免免疫排斥的技术相结合，我们期望开发出一种在较短时间内在鲑鱼中产生精子的实用方法。
Sexually mature individuals are indispensable for breeding programs. Salmonids require a long period before reaching sexual maturity, so we aimed to shorten the period required to obtain functional sperm by grafting immature testicular fragments into mature recipients, which we predicted would allow the grafted testicular fragments to skip the long pre-pubertal period. First, we demonstrated successful subcutaneous auto-grafting of testicular fragments in rainbow trout. Unilateral testectomy was performed, and the isolated immature testicular fragment was auto-grafted into the subcutaneous space along the back of recipient fish. The grafted testicular fragments developed synchronously with the recipients' testis remaining in its body cavity, and both eventually produced functional sperm. Next, immature testicular fragments were auto-grafted into the subcutaneous space of sexually mature males. We achieved this, without immune rejection, by isolating and cryopreserving testes from immature fish, and rearing these unilaterally testectomized fish until sexual maturity. The cryopreserved testes were then auto-grafted into the original, now spermiating fish. The grated immature testicular fragments differentiated and produced functional sperm within 5 months after grafting. By combining this grafting method with a technique to avoid immune rejection, we expect to develop a practical method for producing sperm in a shorter period in salmonids.