Cell-Laden Nanocellulose/Chitosan-Based Bioinks for 3D Bioprinting and Enhanced Osteogenic Cell Differentiation
Cell-Laden Nanocellulose/Chitosan-Based Bioinks for 3D Bioprinting and Enhanced Osteogenic Cell Differentiation
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DOI：
10.1021/acsabm.0c01108
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发表时间：
2021-02-17
影响因子：
4.7
通讯作者：
Benhabbour, S. Rahima
中科院分区：
文献类型：
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作者：
Maturavongsadit, Panita;Narayanan, Lokesh Karthik;Benhabbour, S. Rahima
3D生物打印最近成为组织工程和再生医学中非常有用的工具。然而，开发合适的生物墨水来制造特定的组织构建体仍然是一项具有挑战性的任务。在本文中，我们报告了一种基于纳米纤维素/壳聚糖的生物墨水，该生物墨水与基于3D挤出的生物打印技术兼容，以设计和工程化骨组织工程和再生应用的结构。使用热凝胶化壳聚糖、甘油磷酸盐、羟乙基纤维素和纤维素纳米晶体（CNC）制备生物墨水。通过改变甘油磷酸盐（80-300 mM）、羟乙基纤维素（0-0.5 mg/mL）和CNC（0- 2%w/v）的浓度来优化制剂，以促进快速胶凝动力学（
3D bioprinting has recently emerged as a very useful tool in tissue engineering and regenerative medicine. However, developing suitable bioinks to fabricate specific tissue constructs remains a challenging task. Herein, we report on a nanocellulose/chitosan-based bioink, which is compatible with a 3D extrusion-based bioprinting technology, to design and engineer constructs for bone tissue engineering and regeneration applications. Bioinks were prepared using thermogelling chitosan, glycerophosphate, hydroxyethyl cellulose, and cellulose nanocrystals (CNCs). Formulations were optimized by varying the concentrations of glycerophosphate (80-300 mM), hydroxyethyl cellulose (0-0.5 mg/mL), and CNCs (0-2% w/v) to promote fast gelation kinetics (