Characterization of osteocalcin (BGP) and matrix Gla protein (MGP) fish specific antibodies: validation for immunodetection studies in lower vertebrates.
Characterization of osteocalcin (BGP) and matrix Gla protein (MGP) fish specific antibodies: validation for immunodetection studies in lower vertebrates.
复制标题
骨钙素 (BGP) 和基质 Gla 蛋白 (MGP) 鱼类特异性抗体的表征：低等脊椎动物免疫检测研究的验证。
DOI：
10.1007/s00223-003-0079-4
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Cancela,ML
中科院分区：
文献类型：
--
作者：
Simes,DC;Williamson,MK;Schaff,BJ;Gavaia,PJ;Ingleton,PM;Price,PA;Cancela,ML
In fish species the basic mechanisms of bone development and bone remodeling are not fully understood. The classification of bone tissue in teleosts as cellular or acellular and the presence of transitional states between bone and cartilage and the finding of different types of cartilage in teleosts not previously recognized in higher vertebrates emphasizes the need for a study on the accumulation of the Gla-containing proteins MGP and BGP at the cellular level. In the present study, polyclonal antibodies developed against BGP and MGP fromA. regius(a local marine teleost fish) and against MGP fromG. galeus(a Pacific Ocean shark), were tested by Western blot for their specificity against BGP and MGP from several other species of teleost fish and shark. For this purpose we extracted and purified both proteins from various marine and freshwater teleosts, identified them by N-terminal amino acid sequence analysis and confirmed the presence of gamma-carboxylation in the proteins with the use of a stain specific for Gla residues. Each antibody recognized either BGP or MGP with no cross-reaction between proteins detected. All purified fish BGPs and MGPs tested were shown to be specifically recognized, thus validating the use of these antibodies for further studies.
