Evolution of the plasma proteome of divers before and after a single SCUBA dive
Evolution of the plasma proteome of divers before and after a single SCUBA dive
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DOI：
10.1002/prca.201700016
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发表时间：
2017-09-01
影响因子：
2
通讯作者：
Guerrero, Francois
中科院分区：
文献类型：
--
作者：
Lautridou, Jacky;Pichereau, Vianney;Guerrero, Francois
目的：减压病（DCS）是一种人们知之甚少的复杂全身性疾病，是由于环境压力降低后去饱和度不足引起的。我们之前的一项蛋白质组学研究表明，DCS 的发生（而非潜水）与大鼠血浆蛋白质组的变化有关，包括运甲状腺素蛋白 (TTR) 四聚体丰度的急剧下降。本研究旨在评估引起显着减压压力但不引起 DCS 症状的潜水对人类血液蛋白质组的影响。实验设计：12 名健康男性潜水员在 18 m 海水 (msw) 深度进行单次潜水，在水底停留 47 分钟，然后以 9 msw/min 的速度直接上升到水面。潜水前以及潜水后 30 分钟和 2 小时采集静脉血。然后使用基于二维电泳的蛋白质组策略对四个个体的血浆蛋白质组进行了分析。结果：没有蛋白质点显示丰度发生显着变化（fdr
Purpose: Decompression sickness (DCS) is a poorly understood and complex systemic disease caused by inadequate desaturation following a reduction of ambient pressure. A previous proteomic study of ours showed that DCS occurrence but not diving was associated with changes in the plasma proteome in rats, including a dramatic decrease of abundance of the tetrameric form of Transthyretin (TTR). The present study aims to assess the impact on the human blood proteome of a dive inducing significant decompression stress but without inducing DCS symptoms.Experimental design: Twelve healthy male divers were subjected to a single dive at a depth of 18 m of sea water (msw) with a 47-min bottom time followed by a direct ascent to the surface at a rate of 9 msw/min. Venous blood was collected before the dive as well as 30 min and 2 h following the dive. The plasma proteomes from four individuals were then analyzed by using a two-dimensional electrophoresis-based proteomic strategy.Results: No protein spot showed a significantly changed abundance (fdr