Caspase-2L, Caspase-9, and Caspase-3 During In Vitro Maturation and Fragmentation of the Mouse Oocyte
Caspase-2L, Caspase-9, and Caspase-3 During In Vitro Maturation and Fragmentation of the Mouse Oocyte
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DOI：
10.1002/dvdy.21793
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发表时间：
2008-12-01
影响因子：
2.5
通讯作者：
Lefevre, Brigitte
中科院分区：
文献类型：
--
作者：
Arnault, Emilie;Tosca, Lucie;Lefevre, Brigitte
一些研究表明，凋亡途径控制未受精的排卵卵母细胞的碎片，诱导阿霉素。但很少有人研究过这一过程的基础，从第一阶段的前期到后期阶段。我们的研究结果表明，在减数分裂恢复前，卵母细胞中存在caspase-2（L），caspase-9和caspase-3的酶原和裂解形式。Caspase-2（L）和Caspase-9在GV-卵母细胞核中的分布与染色质构型有关。Z-VAD-fetoxin抑制caspase的活性，加速了减数分裂中期Ⅰ向中期Ⅱ的转变，并在纺锤体中检测到沿着caspase-9和caspase-3的表达。令人惊讶的是，蛋白质印迹分析显示，三个裂解的半胱天冬酶存在于健康和破碎的卵母细胞和半胱天冬酶抑制剂中的类似量并没有阻止阿霉素诱导的细胞凋亡。我们的研究结果表明，如果切割，半胱天冬酶可能会导致最终的卵母细胞死亡，他们可能参与调节成熟过程。发展动力学237：3892-3903，2008年。(c)2008 Wiley-Liss，Inc.
Several studies have shown that apoptotic pathways control fragmentation of unfertilized ovulated oocyte, induced by doxorubicin. But very few have investigated the basis of this process, from prophase I to later stages. Our results revealed the presence of caspase-2(L), caspase-9, and caspase-3 in their zymogen and cleaved forms in the oocyte before meiosis resumption. Caspase-2(L) and caspase-9 were detected in the nucleus of GV-oocytes in a distribution related to chromatin configuration. The inhibition of caspase activity by Z-VAD-fmk accelerated the transition from metaphase I to metaphase II, and caspase-9 and caspase-3 were detected along the meiotic spindle. Surprisingly, Western blot analysis revealed that the three cleaved caspases were present in similar amounts in healthy and fragmented oocytes and caspase inhibition did not prevent doxorubicin-induced apoptosis. Our results suggest that, if cleaved, caspases may be dispensable for final oocyte death and they could be involved in regulating the maturation process. Developmental Dynamics 237:3892-3903, 2008. (c) 2008 Wiley-Liss, Inc.