Facile preparation of multifunctional uniform magnetic microspheres for T-1-T-2 dual modal magnetic resonance and optical imaging
Facile preparation of multifunctional uniform magnetic microspheres for T-1-T-2 dual modal magnetic resonance and optical imaging
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轻松制备用于T-1-T-2双模态磁共振和光学成像的多功能均匀磁性微球
DOI：
10.1016/j.colsurfb.2016.04.014
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Xu Haibo
中科院分区：
文献类型：
--
作者：
Zhang Li;Liang Shuang;Liu Ruiqing;Yuan Tianmeng;Zhang Shulai;Xu Zushun;Xu Haibo
分子成像对于癌症的早期发现和诊断具有重要意义。为此，采用一锅法无乳化剂乳液聚合法制备了一种新型核壳双模式磁共振成像和光学成像磁性微球，在提供高分辨率组织结构信息的同时，实现了高灵敏度的检测。合成的磁性微球由油酸(OA)和十一烷基钠(NaUA)修饰的Fe3O4纳米粒子与苯乙烯(St)、甲基丙烯酸缩水甘油酯(GMA)和可聚合的稀土络合物(Gd(AA)3Phen和Eu(AA)3Phen)在外壳表面聚合而成。荧光光谱显示Eu3+在590 nm和615 nm处有特征发射峰，在双光子共聚焦扫描激光显微镜下可以观察到鲜艳的红色荧光。在基于MTT法的体外细胞毒性测试中，基于MTT法的体外细胞毒性测试表明，在临床3.0TMR扫描下，复合材料的纵向弛豫值(R1)为8.39m m−1s−1，横向弛豫度值(R2)为71.18m m−1s−1。这些令人着迷的多功能特性表明，聚合物微球作为多模式磁共振/光学探针具有巨大的临床潜力。
Molecular imaging is of significant importance for early detection and diagnosis of cancer. Herein, a novel core-shell magnetic microsphere for dual modal magnetic resonance imaging (MRI) and optical imaging was produced by one-pot emulsifier-free emulsion polymerization, which could provide high resolution rate of histologic structure information and realize high sensitive detection at the same time. The synthesized magnetic microspheres composed of cores containing oleic acid (OA) and sodium undecylenate (NaUA) modified Fe3O4nanoparticles and styrene (St), Glycidyl methacrylate (GMA), and polymerizable lanthanide complexes (Gd(AA)3Phen and Eu(AA)3Phen) polymerized on the surface for outer shells. Fluorescence spectra show characteristic emission peaks from Eu3+at 590 nm and 615 nm and vivid red fluorescence luminescence can be observed by 2-photon confocal scanning laser microscopy (CLSM).In vitrocytotoxicity tests based on the MTT assay demonstrate good cytocompatibility, the composites have longitudinal relaxivity value (r1) of 8.39 mM−1s−1and also have transverse relaxivity value (r2) of 71.18 mM−1s−1at clinical 3.0TMR scanner.In vitroandin vivoMRI studies exhibit high signal enhancement on bothT1- andT2-weighted MR images. These fascinating multifunctional properties suggest that the polymer microspheres have large clinical potential as multi-modal MRI/optical probes.