Human cytomegalovirus IE1 protein alters the higher-order chromatin structure by targeting the acidic patch of the nucleosome
Human cytomegalovirus IE1 protein alters the higher-order chromatin structure by targeting the acidic patch of the nucleosome
复制标题
人巨细胞病毒 1E1 蛋白通过靶向核小体的酸性斑块改变高级染色质结构
DOI：
10.7554/elife.11911
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发表时间：
2016-01-26
期刊：
影响因子：
7.7
通讯作者：
Xu, Rui-Ming
中科院分区：
文献类型：
--
作者：
Fang, Qianglin;Chen, Ping;Xu, Rui-Ming
人巨细胞病毒（hCMV）即时早期1 （IE1）蛋白与宿主细胞有丝分裂过程中的浓缩染色质相关。我们已经确定了IE1与核小体核心颗粒结合的染色质系聚域（CTD）的结构，并发现IE1-CTD与H2A-H2B酸性斑块之间的特异性相互作用损害了高阶染色质结构的压实。我们的研究结果表明，在hCMV感染期间，IE1使宿主染色质的折叠松弛。
Human cytomegalovirus (hCMV) immediate early 1 (IE1) protein associates with condensed chromatin of the host cell during mitosis. We have determined the structure of the chromatin-tethering domain (CTD) of IE1 bound to the nucleosome core particle, and discovered that the specific interaction between IE1-CTD and the H2A-H2B acidic patch impairs the compaction of higher-order chromatin structure. Our results suggest that IE1 loosens up the folding of host chromatin during hCMV infections.