Immunohistochemical demonstration of oestrogen and progesterone receptors: correlation of standards achieved on in house tumours with that achieved on external quality assessment material in over 150 laboratories from 26 countries
Immunohistochemical demonstration of oestrogen and progesterone receptors: correlation of standards achieved on in house tumours with that achieved on external quality assessment material in over 150 laboratories from 26 countries
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DOI：
10.1136/jcp.53.4.292
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发表时间：
2000-04-01
影响因子：
3.4
通讯作者：
Miller, KD
中科院分区：
文献类型：
--
作者：
Rhodes, A;Jasani, B;Miller, KD
为了研究免疫组化（IHC）检测雌激素受体（ER）和孕激素受体（PR）的敏感性，实验室对乳腺肿瘤进行固定和处理，并将其与作为外部质量评估（EQA）计划的一部分而传播的谣言的敏感程度进行比较。将显示不同程度ER和PR表达的乳腺癌组织切片循环到参与英国国家免疫细胞化学外部质量评估方案（UKNEQAS-ICC）的实验室进行IHC染色。这些谣言的染色，除了在参与者自己的实验室中固定和处理的肿瘤外（锡屋谣言），由一个由四名评估员组成的小组使用既定的英国NEQAS-ICC评分系统进行评估。对于一个选定的评估运行，参与者在房子肿瘤的表达程度进行了评估，通过半定量快速score method.Results-Although授予的分数染色的内部谣言一般高于授予的染色的英国NEQAS肿瘤，也有一个显着的正相关性两组分数之间。使用快速评分法对其中一次评估运行进行评估，17%的内部肿瘤被归类为具有高度ER表达。在其余的病例中，最初被分类为仅具有低或中等ER表达的显著比例在组织实验室染色时被发现具有较高的表达。英国NEQAS-ICC中心的激素受体的常规检测被发现是90-100%的效率，在实现最佳的演示乳腺肿瘤从超过150个不同的laborators. Conclusions，英国NEQAS肿瘤和在内部的谣言获得的结果之间的显着正相关性提供证据的观点认为，EQA材料上取得的结果是在内部实验室性能的准确指标。虽然大多数实验室充分检测肿瘤高受体表达，大部分的内部肿瘤最初分类的参与者的染色为低或中等ER表达有较高程度的表达时，由英国NEQAS-ICC中心染色。组织中心的ER和PR的常规INC方法在最佳展示参与者在内部乳房谣言的效率表明，固定和组织制备的变化是不妨碍不同的实验室实现最佳演示的限制因素。
To investigate the sensitivity of immunohistochemical (IHC) assays for oestrogen receptors (ER) and progesterone receptors (PR) achieved by laboratories on breast tumours fixed and processed in their own department, and to compare this with the degree of sensitivity they achieve on rumours circulated as part of an external quality assessment (EQA) programme.Methods-On 10 occasions between April 1994 and June 1998, histological sections from breast cancers showing various degrees of expression of ER and PR were circulated for IHC staining to laboratories participating in the UK national external quality assessment scheme for immunocytochemistry (UK NEQAS-ICC). The staining of these rumours, in addition to that of tumours fixed and processed in the participants own laboratories tin house rumours), was assessed by a panel of four assessors, using the established UK NEQAS-ICC scoring system. For a selected assessment run, the degree of expression of participants in house tumours was evaluated by means of the semiquantitative quick score method.Results-Although the scores awarded for the staining of in house rumours were generally higher than those awarded for the staining of UK NEQAS tumours, there was also a significant positive correlation between the two sets of scores. Using the quick score method of evaluation for one of the assessment runs, 17% of in house tumours were classified as having a high degree of ER expression. Of the remaining cases, a significant proportion initially classified as having only low or medium expression of ER were found to have higher expression when stained by the organising laboratory. The UK NEQAS-ICC centre's routine assay for hormonal receptors was found to be 90-100% efficient in achieving optimal demonstration of breast tumours from over 150 different laboratories.Conclusions-The significant positive correlation between the results obtained on the UK NEQAS tumours and the in house rumours provides evidence for the view that results achieved on EQA material are accurate indicators of in house laboratory performance. Although most laboratories adequately detected tumours with high receptor expression, a large proportion of in house tumours classified initially by participants' staining as being of low or medium ER expression had a higher degree of expression when stained by the UK NEQAS-ICC centre. The efficiency of the organising centre's routine INC method for ER and PR in optimally demonstrating participants in house breast rumours shows that variations in fixation and tissue preparation are not limiting factors preventing a different laboratory achieving optimal demonstration.