Vertical transmission of bovine spongiform encephalopathy prions evaluated in a transgenic mouse model
Vertical transmission of bovine spongiform encephalopathy prions evaluated in a transgenic mouse model
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DOI：
10.1128/jvi.79.13.8665-8668.2005
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发表时间：
2005-07-01
影响因子：
5.4
通讯作者：
Torres, JM
中科院分区：
文献类型：
--
作者：
Castilla, J;Brun, A;Torres, JM
在这项工作中，我们显示的证据表明，在一个转基因小鼠系表达牛朊蛋白（boTg）实验感染的牛海绵状脑病（BSE）朊病毒的脑内管理的母婴传播。只有当交配被允许接近疾病的临床阶段，当脑PrPres沉积可以通过Western印迹分析检测到，从感染的母亲的新生儿的大脑中检测到PrPres。在boTg小鼠脑内接种后，试图检测牛奶中的感染性是不成功的，这表明其他组织作为朊病毒传播载体的参与。这里显示的结果证明了BSE朊病毒从中枢神经系统向外周组织和小鼠模型后代离心扩散的能力。此外，这些结果可以补充以前的流行病学数据支持牛疯牛病垂直传播的发生。
In this work we show evidence of mother-to-offspring transmission in a transgenic mouse line expressing bovine PrP (boTg) experimentally infected by intracerebrall administration of bovine spongiform encephalopathy (BSE) prions. PrPres was detected in brains of newborns from infected mothers only when mating was allowed near to the clinical stage of disease, when brain PrPres deposition could be detected by Western blot analysis. Attempts to detect infectivity in milk after intracerebral inoculation in boTg mice were unsuccessful, suggesting the involvement of other tissues as carriers of prion dissemination. The results shown here prove the ability of BSE prions to spread centrifugally from the central nervous system to peripheral tissues and to offspring in a mouse model. Also, these results may complement previous epidemiological data supporting the occurrence of vertical BSE transmission in cattle.