Isolation of variants in phagocytosis of a macrophage-like continuous cell line.
Isolation of variants in phagocytosis of a macrophage-like continuous cell line.
复制标题
分离巨噬细胞状连续细胞系的吞噬作用中变体的分离。
DOI：
10.1084/jem.145.1.175
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发表时间：
1977-01-01
影响因子：
15.3
通讯作者：
Bloom, B R
中科院分区：
文献类型：
--
作者：
Muschel, R J;Rosen, N;Bloom, B R
我们已经从克隆的连续小鼠巨噬细胞样细胞系J 774.2中分离出吞噬作用的克隆变体。使用含有毒性药物杀结核菌素的IgG包被SRBC作为致死剂，设计了针对Fc受体介导的吞噬作用的选择程序。分离了一系列Fc受体介导的吞噬作用缺陷的变体克隆。这些变异体发生频率较低（约6 × 10 - 5），稳定，似乎具有Fc受体。它们在IgG包被的SRBC的吞噬作用中的缺陷程度因克隆而异，但所有克隆都能够吞噬乳胶颗粒。在某些变体中的吞噬缺陷可以通过添加8 Br-cAMP来纠正，在其他变体中，药物没有效果。因此，很可能不同的变体在Fc受体介导的吞噬作用的几个关键的不同步骤中是有缺陷的。
We have isolated cloned variants in phagocytosis from a cloned continuous murine macrophage-like cell line, J 774.2. A selection procedure against Fc receptor-mediated phagocytosis was devised using IgG-coated SRBC containing a toxic drug, tubercidin, as the lethal agent. A series of variant clones deficient in Fc receptor-mediated phagocytosis were isolated. Such variants occurred at low frequency (approximately 6 X 10(-5)), were stable, and appeared to possess Fc receptors. The degree to which they were defective in phagocytosis of IgG-coated SRBC varied from clone to clone, yet all clones, were able to phagocytize latex particles. The phagocytic defect in some variants could be corrected by the addition of 8 Br-cAMP, in others, the drug was without effect. It is likely, therefore, that different variants are defective in several distinct steps critical to Fc receptor- mediated phagocytosis.