Nematicidal Activity of Chaetoglobosin A Poduced by Chaetomium globosum NK102 against Meloidogyne incognita
Nematicidal Activity of Chaetoglobosin A Poduced by Chaetomium globosum NK102 against Meloidogyne incognita
复制标题
球毛壳素 NK102 对南方根结线虫的杀线虫活性
DOI：
10.1021/jf304314g
复制
发表时间：
2013-01-09
影响因子：
6.1
通讯作者：
Zhu, Xudong
中科院分区：
文献类型：
--
作者：
Hu, Yang;Zhang, Weipu;Zhu, Xudong
对球毛壳菌NK102、其培养滤液以及通过高效液相色谱法纯化的球毛壳菌素A（ChA）对南方根结线虫的杀线虫活性进行了评估。结果表明，球毛壳菌NK102显著排斥二期幼虫（J2）。滤液和ChA对J2死亡率均表现出强烈的不利影响，在72小时时，300μg ChA/mL浓度下死亡率为99.8%（半数致死浓度LC50 = 77.0μg/mL）。ChA和滤液在暴露72小时前不影响卵孵化。所有滤液处理即使在12.5%稀释处理下也抑制J2侵入。同样，ChA（300和30μg/mL）对J2侵入显示出显著的抑制作用。与对照植株相比，30mg ChA/kg土壤处理中每株植物的卵数量显著减少了63%，这表明对南方根结线虫的繁殖有明显的负面影响。该研究证明了ChA的杀线虫活性，并表明它可能是南方根结线虫综合管理的一种潜在生物防治剂。
The nematicidal activity of Chaetomium globosum NK102, culture filtrates, and chaetoglobosin A (ChA) purified by HPLC was evaluated on Meloidogyne incognita. The results showed that C. globosum NK102 significantly repelled second-stage juveniles (J2s). Both filtrates and ChA demonstrated strong adverse effects on J2 mortality with 99.8% at 300 mu g ChA/mL (LC50 = 77.0 mu g/mL) at 72 h. ChA and filtrates did not affect egg hatch until 72 h of exposure. All filtrate treatments inhibited the J2 penetration even in 12.5% dilution treatment. Similarly, ChA (300 and 30 mu g/mL) showed a significant inhibitory effect on J2 penetration. The number of eggs per plant was significantly reduced in the treatment of 30 mg ChA/kg soil by 63% relative to control plants, indicating the apparent negative effect on reproduction of M. incognita. The study demonstrated the nematicidal activity of ChA and suggested that it could be a potential biocontrol agent for integrated management of M. incognita.