Progenitors for Ly-1 B cells are distinct from progenitors for other B cells.
Progenitors for Ly-1 B cells are distinct from progenitors for other B cells.
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DOI：
10.1084/jem.161.6.1554
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发表时间：
1985-06-01
影响因子：
15.3
通讯作者：
Herzenberg, L A
中科院分区：
文献类型：
--
作者：
Hayakawa, K;Hardy, R R;Herzenberg, L A;Herzenberg, L A
来自以前的多参数荧光激活细胞分选仪(FACS)分析和分选研究的数据定义了表达Ly-1表面决定簇与IgM、IGD、Ia和其他典型B细胞标记物的小鼠B细胞的子集。这些Ly-1B细胞在物理和功能上是不同的。与大多数其他B细胞相比，它们表达更多的IgM和更少的IgD；它们通常不存在于淋巴或骨髓中；它们在正常脾中的出现频率总是很低(1-5%)，正如我们在这里所示，它们约占正常小鼠从腹膜腔中回收的B细胞的一半(占总细胞的10%-20%)。此外，在正常和自身免疫的小鼠中，通常研究的大多数IgM自身抗体都是由这些Ly-1B细胞产生的，尽管它们很少产生针对外源性抗原的抗体，如三硝基苯基菲科尔或三硝基苯基孔帽状血蓝蛋白。本文介绍的细胞转移研究表明，Ly-1B细胞的祖细胞不同于脾和淋巴结中主要B细胞群体的祖细胞。在这些研究中，我们使用流式细胞仪分析和功能分析来表征移植后1-2个月致死性照射受者体内存在的供者来源的(同种异型标记)B细胞。令人惊讶的是，通常用于重建辐射受者B细胞的成人骨髓细胞选择性地未能重建Ly-1B亚群。相反，幼年小鼠的肝、脾和骨髓细胞重组了所有的B细胞(包括Ly-1B)，而成年小鼠的腹膜“洗脱”细胞(Perc)独特地重组了Ly-1B。骨髓并没有阻止Ly-1B的发育，因为当Perc和新生的肝脏与骨髓联合转移时，仍然可以产生Ly-1B。这些发现初步将Ly-1B归属于一个不同的发育谱系，其起源于幼年动物中与其他B细胞前体细胞相同位置的祖细胞，但在成年动物中转移到独特的位置(S)。
Data from previous multiparameter fluorescence-activated cell sorter (FACS) analysis and sorting studies define a subset of murine B cells that expresses the Ly-1 surface determinant in conjunction with IgM, IgD, Ia, and other typical B cell markers. These Ly-1 B cells are physically and functionally distinct. They express more IgM and less IgD than most other B cells; they are not normally found in lymph node or bone marrow; they are always present at low frequencies (1-5%) in normal spleens, and, as we show here, they comprise about half of the B cells (10-20% of total cells) recovered from the peritoneal cavity in normal mice. Furthermore, most of the commonly studied IgM autoantibodies in normal and autoimmune mice are produced by these Ly-1 B cells, even though they seldom produce antibodies to exogenous antigens such as trinitrophenyl-Ficoll or trinitrophenyl-keyhole limpet hemocyanin. Cell transfer studies presented here demonstrate that the progenitors of Ly-1 B cells are different from the progenitors of the predominant B cell populations in spleen and lymph node. In these studies, we used FACS analysis and functional assays to characterize donor-derived (allotype-marked) B cells present in lethally irradiated recipients 1-2 mo after transfer. Surprisingly, adult bone marrow cells typically used to reconstitute B cells in irradiated recipients selectively failed to reconstitute the Ly-1 B subset. Liver, spleen, and bone marrow cells from young mice, in contrast, reconstituted all B cells (including Ly-1 B), and peritoneal "washout" cells (PerC) from adult mice uniquely reconstituted Ly-1 B. Bone marrow did not block Ly- 1 B development, since PerC and newborn liver still gave rise to Ly-1 B when jointly transferred with marrow. These findings tentatively assign Ly-1 B to a distinct developmental lineage originating from progenitors that inhabit the same locations as other B cell progenitors in young animals, but move to unique location(s) in adults.