Plant Sterol Clustering Correlates with Membrane Microdomains as Revealed by Optical and Computational Microscopy.
Plant Sterol Clustering Correlates with Membrane Microdomains as Revealed by Optical and Computational Microscopy.
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光学和计算显微镜揭示植物甾醇聚类与膜微域相关
DOI：
10.3390/membranes11100747
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发表时间：
2021-09-29
期刊：
影响因子：
4.2
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Tang L;Li Y;Zhong C;Deng X;Wang X
脂质组成的局部不均匀性在信号转导和膜运输的调节中起着至关重要的作用。对于植物质膜尤其如此，其富含特定脂质，例如游离和缀合形式的植物甾醇和典型的植物鞘脂。然而，大多数细胞中微区的证据仍然是间接的，膜不均匀性的性质一直难以表征。我们使用了一种新的推拉芘探针和荧光寿命成像显微镜（FLIM）结合全原子多尺度分子动力学模拟磷脂和植物甾醇之间的相互作用和调节细胞植物甾醇对膜相关的微区和相分离形成的影响提供了详细的视图。我们对生物膜的组织原理的理解主要受限于测量活细胞复杂环境中脂质和蛋白质的分布和相互作用的挑战。比较典型的液体无序（Ld）和液体有序（Lo）域的磷脂/植物甾醇组合物，我们进一步表明，植物甾醇在膜稳态中起着至关重要的作用。模拟工作突出了最先进的建模如何消除了一些先前的担忧，以及如何通过计算显微镜进行无可辩驳的发现。总之，我们的研究结果支持植物甾醇在植物细胞PM的横向结构中的作用，并表明它们是形成植物PM微区和脂质有序相的关键化合物。
Local inhomogeneities in lipid composition play a crucial role in the regulation of signal transduction and membrane traffic. This is particularly the case for plant plasma membrane, which is enriched in specific lipids, such as free and conjugated forms of phytosterols and typical phytosphingolipids. Nevertheless, most evidence for microdomains in cells remains indirect, and the nature of membrane inhomogeneities has been difficult to characterize. We used a new push–pull pyrene probe and fluorescence lifetime imaging microscopy (FLIM) combined with all-atom multiscale molecular dynamics simulations to provide a detailed view on the interaction between phospholipids and phytosterol and the effect of modulating cellular phytosterols on membrane-associated microdomains and phase separation formation. Our understanding of the organization principles of biomembranes is limited mainly by the challenge to measure distributions and interactions of lipids and proteins within the complex environment of living cells. Comparing phospholipids/phytosterol compositions typical of liquid-disordered (Ld) and liquid-ordered (Lo) domains, we furthermore show that phytosterols play crucial roles in membrane homeostasis. The simulation work highlights how state-of-the-art modeling alleviates some of the prior concerns and how unrefuted discoveries can be made through a computational microscope. Altogether, our results support the role of phytosterols in the lateral structuring of the PM of plant cells and suggest that they are key compounds for the formation of plant PM microdomains and the lipid-ordered phase.
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影响因子：
3
作者：
Kutzner, Carsten;Pall, Szilard;Fechner, Martin;Esztermann, Ansgar;de Groot, Bert L.;Grubmueller, Helmut
通讯作者：
Grubmueller, Helmut
影响因子：
3.4
作者：
Castillo, Norberto;Monticelli, Luca;Tieleman, D. Peter
通讯作者：
Tieleman, D. Peter
影响因子：
3.4
作者：
Ge, Yifan;Gao, Jiayun;Naumann, Christoph A.
通讯作者：
Naumann, Christoph A.
影响因子：
4.8
作者：
Castell, Oliver K.;Dijkman, Patricia M.;Goddard, Alan D.
通讯作者：
Goddard, Alan D.
影响因子：
3.9
作者：
Flasinski, Michal;Wydro, Pawel;Fontaine, Philippe
通讯作者：
Fontaine, Philippe