Quantitative PCR tissue expression profiling of the human SGLT2 gene and related family members.
Quantitative PCR tissue expression profiling of the human SGLT2 gene and related family members.
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DOI：
10.1007/s13300-010-0006-4
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发表时间：
2010-12
期刊：
影响因子：
3.8
通讯作者：
Feder, John N
中科院分区：
文献类型：
--
作者：
Chen, Jian;Williams, Sandy;Ho, Samantha;Loraine, Howard;Hagan, Deborah;Whaley, Jean M;Feder, John N
SGLT2(钠葡萄糖共转运体蛋白2)是肾脏中负责葡萄糖重吸收的主要蛋白质，其抑制作用一直是治疗2型糖尿病药物开发工作的重点。为了更好地阐明SGLT2及其相关辅转运体成员在人体组织中的表达分布，我们对来自三个不同个体的72个正常组织的RNA进行了TaqMan™(应用生物系统公司，美国加利福尼亚州福斯特市)的定量聚合酶链式反应分析。我们一直观察到SGLT2是高度肾脏特异性的，而SGLT5是肾脏高度丰富的；SGLT1、钠依赖氨基酸转运体(SAAT1)和SGLT4在小肠和骨骼肌中高度丰富；SGLT6在中枢神经系统表达；钠肌醇共转运体在人体所有组织中普遍表达。本文的补充材料可通过10.1007/s133000100006-4获得，授权用户可访问。
SGLT2 (for “Sodium GLucose coTransporter” protein 2) is the major protein responsible for glucose reabsorption in the kidney and its inhibition has been the focus of drug discovery efforts to treat type 2 diabetes. In order to better clarify the human tissue distribution of expression of SGLT2 and related members of this cotransporter class, we performed TaqMan™ (Applied Biosystems, Foster City, CA, USA) quantitative polymerase chain reaction (PCR) analysis of SGLT2 and other sodium/glucose transporter genes on RNAs from 72 normal tissues from three different individuals. We consistently observe that SGLT2 is highly kidney specific while SGLT5 is highly kidney abundant; SGLT1, sodium-dependent amino acid transporter (SAAT1), and SGLT4 are highly abundant in small intestine and skeletal muscle; SGLT6 is expressed in the central nervous system; and sodium myoinositol cotransporter is ubiquitously expressed across all human tissues. Supplementary material is available for this article at 10.1007/s13300-010-0006-4 and is accessible for authorized users.