Establishment of a system to quantify wild‐type herpes simplex virus-induced cell-cell fusion reveals a role of N‐glycosylation of HSV‐1 envelope glycoprotein B in cell?cell fusion
Establishment of a system to quantify wild‐type herpes simplex virus-induced cell-cell fusion reveals a role of N‐glycosylation of HSV‐1 envelope glycoprotein B in cell?cell fusion
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野生型单纯疱疹病毒诱导的细胞融合定量系统的建立揭示了 HSV-1 包膜糖蛋白 B N-糖基化在细胞融合中的作用
DOI：
10.1111/1348-0421.13050
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发表时间：
2023
影响因子：
2.6
通讯作者：
Kawaguchi Yasushi
中科院分区：
文献类型：
--
作者：
Fukui Ayano;Maruzuru Yuhei;Takeshima Kosuke;Koyanagi Naoto;Kato Akihisa;Kawaguchi Yasushi
野生型单纯疱疹病毒（HSV）毒株很少在细胞培养物中介导细胞-细胞融合，几乎不诱导大的多核细胞。在这项研究中，我们建立了一个系统来定量野生型HSV毒株诱导的罕见细胞-细胞融合。所建立的系统阐明了HSV-1包膜糖蛋白B及其在位置141处的天冬酰胺处的N-糖基化是有效的细胞-细胞融合所必需的。这项研究提供了野生型HSV-1诱导的细胞-细胞融合与体内病毒发病机制之间的联系。
Wild‐type herpes simplex virus (HSV) strains infrequently mediate cell–cell fusion in cell cultures and barely induce large multinucleated cells. In this study, we established a system to quantify infrequent cell–cell fusion induced by wild‐type HSV strains. The established system clarified that the HSV‐1 envelope glycoprotein B and its N‐glycosylation at asparagine at position 141 were required for efficient cell–cell fusion. This study provides a link between cell–cell fusion induced by wild‐type HSV‐1 and viral pathogenesis in vivo.