Selective expression of a subset of neuronal genes in oligodendroglioma with chromosome 1p loss
Selective expression of a subset of neuronal genes in oligodendroglioma with chromosome 1p loss
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DOI：
10.1111/j.1750-3639.2004.tb00495.x
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发表时间：
2004-01-01
期刊：
影响因子：
6.4
通讯作者：
Aburatani, H
中科院分区：
文献类型：
--
作者：
Mukasa, A;Ueki, K;Aburatani, H
胶质瘤的分类主要基于其与假定的胶质细胞起源的显微相似性，如星形胶质细胞和少突胶质细胞。然而，更客观的诊断标准对于患者的精确治疗是必不可少的。例如，少突胶质细胞中1号染色体短臂（1p）的缺失被认为是对化疗反应更好、患者生存时间更长的重要标志。为了深入了解它们的分子生物学背景，并确定表征每个亚群的基因，我们使用DNA微阵列研究了4个胶质瘤亚群的基因表达谱，这些亚群分别是有和没有1p缺失的少突胶质细胞瘤、弥漫性星形细胞瘤和胶质母细胞瘤。值得注意的是，在1p缺失少突胶质细胞瘤中表现出独特表达的大部分基因也在正常脑组织中高表达，并具有神经元相关功能，包括MYT1L、INA、RIMS2、SNAP91和SNCB。组织学分析也证实，在正常神经元中大量表达的MYT1L在肿瘤细胞中也存在。这些结果表明，尽管少突胶质细胞瘤被认为起源于胶质谱系细胞，但其或多或少具有神经元特征，尤其是1p缺失。通过进一步的病理研究，这些神经元相关基因也可能成为预后较好的少突胶质细胞瘤的良好诊断标志物。
Gliomas are classified based mainly on microscopic resemblance to their presumed glial origin such as astrocyte and oligodendrocyte. However, more objective diagnostic criteria are indispensable for the precise treatment of patients. For instance, loss of the short arm of chromosome 1 (1p) in oligodendrogliornas is recognized as an important marker for better response to chemotherapy and longer survival of the patients. To gain insight into their molecular biological background and to identify genes characterizing each subgroup, we investigated gene expression profile of the 4 glioma subsets, oligodendroglioma with and without 1p loss, diffuse astrocytoma and glioblastoma using DNA microarray. Remarkably, most of the genes showing distinctive expression in oligodendroglioma with 1p loss were also highly expressed in normal brain tissues and had neuron-related function, which included MYT1L, INA, RIMS2, SNAP91 and SNCB. Histological analysis also demonstrated that MYT1L, which were abundantly expressed in normal neuron, were certainly present in tumor cells. These results suggest that oilgodendroglioma, especially with 1p loss, has more or less neuronal characteristics although oligodendroglioma is thought to originate form glial lineage cell. With further pathological studies, those neuron-related genes might be good diagnostic markers for oligodendroglioma of better prognosis as well.